Polymerase chain reaction degenerate

Daigo Y, Chin S-F, Gorringe KL, et al. Degenerate oligonucleotide primed-polymerase chain reaction-based array comparative genomic hybridization for extensive Amplicon profiling of breast cancers. A new approach for the molecular analysis of paraffin-embedded cancer tissue. Am. J. Pathol. 2001 158 1623-1631. 

Stoecklein NH, Erbersdobler A, Schmidt-Kittler O, et al. SCOMP is superior to degenerated oligonucleotide primed-polymerase chain reaction for global amplification of minute amounts of DNA from microdissected archival tissue samples. Am. J. Pathol. 2002 161 43-51. 

Subacute AIDS encephalitis (K3) was detected in adult and pediatric brains. Immunocytochemical analysis of adult and pediatric brains revealed gp 41 im-munoreactivity (78% and 40% respectively). Virtually all adult brains with SAE had gp 41 immunoreactivity in macrophages and microglia. Spinal cords with vacuolar myelopathy or corticospinal tract degeneration had only rare gp 41 positive cells. Brains of aborted fetuses from HIV-1 seropositive women were negative for gp 41 immunocreactivity, but some were positive for HIV-1 by polymerase chain reaction. 

Cheng, J., Waters, L.C., Fortina, P., Hvichia, G., Jacobson, S.C., Ramsey, J.M., Kricka, L.J., Wilding, P., Degenerate oligonucleotide primed-polymerase chain reaction and capillary electrophoretic analysis of human DNA on microchip-based devices. Anal. Biochem. 1998, 257(2), 101-106. 

K7. Knoth, K., Roberds, S., Poteet, C., and Tamkun, M., Highly degenerate, inosine-containing primers specifically amplify rare cDNA using the polymerase chain reaction. Nucleic Acids Res. 16, 10932 (1988). 

To this end, cDNA libraries, prepared from cells or tissues known to be rich in certain receptors, were screened by low stringency hybridization, or were used for polymerase chain reaction (PCR) amplification of candidate genes using degenerate primers. Proof of function was obtained after the... 

This homology allowed for the design of degenerate primers to search for related proteins using the polymerase chain reaction which lead to the subsequent identification of neurotrophin-3 (NT-3) (Ernfors et al., 1990a Hohn et al., 1990 ... 

The technique was soon complemented by the polymerase chain reaction (PGR), using partially degenerate primers. The PGR strategy has two principal advantages. First, it requires a lower overall degree of DNA sequence conservation. Second, it can use much... 

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