Polymerase chain reaction chromatography

McWhorter S, Soper SA. Conductivity detection of polymerase chain reaction products separated by micro-reversed-phase liquid chromatography. Journal of Chromatography A 883,1-9, 2000. 

Reverse transcription-polymerase chain reaction. Thin layer chromatography. 

Viral detection assays based on infectivity suffer from significant variability, which necessitates the use of statistical evaluation. Polymerase chain reaction-based assays are currently being developed and validated for viral clearance. With PCR assays, there is a potential to distinguish between inactivation and physical removal, perform mass balance studies, evaluate more than one vims at a time for a given process step, reduce the time for completing clearance studies, and accurately quantitate the amount of vims bound to such surfaces as chromatography resins. Table 5 compares the assay precision between an infectivity assay and a quantitative PCR assay. 

SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis HPLC, high performance liquid chromatography PCR, polymerase chain reaction IEF, isoelectric focusing HPSEC, high performance size-exclusion chromatography DNA, DNA-binding fluorochrome CPE, cytopathic effect Had, hemadsorption PMA, production of murine antibodies. 

Lam, C.W. Analysis of polymerase chain reaction products by denaturing high-performance liquid chromatography. Meth. Mol. Biol. 2006, 336, 73-82. 

Reverse phase high-performance liquid chromatography, see RP-HPLC Reverse transcriptase polymerase chain reaction (RT-PCR) 290... 

As pointed out earlier, microfluidic systems have a wide range of applications, e.g. heat exchange systems for electronic devices [28-31], medical diagnostic and analytical chemical applications [69, 70], and precision dilution systems with minimal dead volume for gas chromatography [71]. More may be anticipated as the technology matures. Current research at many laboratories has shown the need to provide flow control at extremely low levels for sensor-controlled implanted drug delivery systems [72] and portable diagnostic cards for polymerase chain reaction analysis [73]. 

Germini A, Scaravelli E, Lesignoli F, Sforza S, Corradini R, Marchelli R (2005). Polymerase chain reaction coupled with peptide nucleic acid high-performance liquid chromatography for the sensitive detection of traces of potentially allergenic hazelnut in foodstuffs. Eur. Food Res. Technol., 220 619-624. 

The use of animal antibodies to detect antigens is highly effective but it involves the use of animals, complex separations, and has a variety of limitations. Starting around 1990 a new method for detection and separation of substances, including amino acids, proteins, and pharmaceuticals, was developed based on synthetic nucleic acids termed aptomers aptos = "to fit"). Aptomers are DNA or RNA molecules that bind with high specificity to certain molecules. Their application depends upon many principles described earlier in this book chromatography, combinatorial chemistry, and the polymerase chain reaction (PCR) technique. 

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