Polydeoxyadenylic acid

Figure 18 Separation of polydeoxyadenylic acid mixture. (Courtesy of Beckman Instruments Fullerton, CA.)...

Smith RA, Williamson DS, Cohen SM. 1988. Detection and characterization of an acrolein adduct in polydeoxyadenylic acid. In 72nd Annual Meeting Federation of American Societies for Experimental Biology, Las Vegas, NV, May 1-5, 1988 [Abstract], Fed Am Soc Exp Biol J 2 4991. 

Figure 10.5.39 HPLC Chromatogram Showing the Separation of 2-9 mers of Polydeoxyadenylic Acids.

Sessler and coworkers have recently developed a high-performance liquid chromatography (HPLC) support with sapphyrin-functionalized silica gel [67]. Oxyanions were found to interact with the sapphyrin unit, and thus they were retained longer compared to an appropriate control support. The separations of adenosine, adenosine 5 -phosphate (AMP), adenosine 5 -diphosphate (ADP) and ATP, and dimers to nonamers of polydeoxyadenylic acids at neutral pH were also achieved. 

First, a simplified model of DNA, polydeoxyadenylic acid, was shown to be readily cleaved at neutral pH by Mn-TMPyP/KHSOs with spontaneous release of free adenine and, after heating, formation of a rather unstable sugar degradation product identified as 5-methylene-2(5H)-furanone, supporting a C-1 oxidation pathway. The first /3-elimination created a 5 -phosphate end and the second one was accompanied by 5-MF release. The two DNA strands were terminated by phosphate groups (Fig. 6, route A) (282). 

Polydeoxyinosinic-deoxycytidylic acid sodium salt Polydeoxyadenylic-thymidylic acid sodium salt Polydeoxyguanylic-deoxycytidylic acid sodium salt Polydeoxyguanylic-polydeoxycytidylic acid sodium salt Polycytidylic-polyguanylic acid sodium salt Polyadenylic-polyuridylic acid sodium salt 8-Oxo-7,8-dihydroguanine Further oxidation products of 8-oxo-G... 

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