Polyamines mutants

The precise role of polyamines in the control of dormancy and the onset of cell division in Jerusalem artichoke tubers, and for that matter in plant development per se, remains in question. Evidence from arginine decarboxylase mutants of Arabidopsis does indicate a possible role in root meristem function (Watson et al., 1998). 

Watson, M.B., Emory, K.K., Piatak, R.M., and Malmberg, R.L., Arginine decarboxylase (polyamine synthesis) mutants of Arabidopsis thaliana exhibit altered root growth, Plant J., 13, 231-239, 1998. 

Jung, I.L., Oh, T.J., and Kim, LG, (2003) Abnormal growth of polyamine-deficient Escherichia coli mutant is partially caused by oxidative stress-induced damage. Arch. Biochem. Biophys., 418 (2), 125-132. 

Low, K.B., and Canellakis, E.S. (1987) Biosynthesis of polyamines in ornithine decarboxylase, arginine decarboxylase, and agmatine ureohydrolase deletion mutants of Escherichia coli strain K-12. Proc. Natl Acad. Sci. USA., 84 (13), 4423 -4427. 

We next looked for protein(s) whose synthesis is stimulated by polyamines at the level of translation using a polyamine-requiring mutant of Saccharomyces cerevi-siae deficient in ornithine decarboxylase (YPH499dspei). When the YPU499Aspel mutant was cultured in polyamine-fi ee medium, spermidine content was negligible (1.1 nmol/mg protein) and the rate of cell growth was delayed. When 0.1 mM... 

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