Poly synthetase purification

The enzyme catalyzing the transfer of D-apiose from UDP-apiose to 4, 5-dihydroxyflavon-7-yl /3-D-glucopyranoside is commonly called apiin synthetase.7 Activity is measured by the formation of [14C]apiin from UDP-[U-14C]apiose. Apiin synthetase can be measured by the rapid separation and isolation, by poly(ethylenimine)-paper chromatography, of a product of the reaction, namely, [14C] apiin, from UDP-D-[U-14C]xylose and degradation products of UDP-[U-14C]apiose.31 There are reports of the isolation and purification of apiin synthetase from parsley leaves,31 from cell-suspension cultures of parsley,121 and from foxglove (Digitalis purpurea).31 Apiin synthetase isolated from parsley does not require metal ions, NAD+, or other cofactors, and is soluble. It is inhibited by several heavy metals, but not by tetra-N-... 

Recent attempts at characterization of the poly(ADP-ribose) synthetase have dealt with localization of the enzyme on chromatin, purification and requirements of synthetase for catalytic function, effects of DNA and histones on activity, and the protein substrates of the synthetase. 

Ito S, Shizuta Y, Hayaishi O (1979) Purification and characterization of poly(ADP-ribose) synthetase from calf thymus. J Biol Chem 254 3647-3651... 

Agemori M, Kagamiyama H, Nishikimi M, Shizuta Y (1982) Purification and properties of poly(ADP-ribose) synthetase from mouse testicle. Arch Biochem Biophys 215 621-627... 

Okayama H, Edson CM, Fukushima M, Ueda K, Hayaishi 0 (1977) Purification and properties of poly(adenosine diphosphate ribose) synthetase. J Biol Chem 252 7000-7005... 

Exciting progress - has been made in the enzymology of systems which elaborate phenolic compounds by the acetate-poly maionate pathway. The purification and properties of 6-MSA (6-methylsalicylic acid) (4) synthetase were reported in 1970 and recent work has provided further information on this enzymic system. Thus it has been reported that an ammonium sulphate protein fraction from P. patulum catalyses the synthesis of 6-MSA, TAL (triacetic acid lactone) (2), and fatty acids in the presence of malonyl-coenzyme A and NADPH. The derivation of 6-MSA from one acetate and three maionate... 

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