Plants wheat germination

Five-g samples of wheat plant material were soaked in 150 ml of water for 10 hr at room temperature. The mixture was filtered and the filtrate used to germinate pitted morningglory (Ipomoea lacunosa L.) and ragweed (Ambrosia artemisiifolia L.) seed. The effect of the extract on germination was tested in the presence and absence of light. 

Over 50 different pyridazin-3-ones were evaluated for biological activity in a wheat (Triticum aestivum L.) test system described previously (1). Briefly, seeds were germinated in 9-cm petri dishes on three layers of filter paper. Pyridazinones were dissolved in acetone and the filter papers were impregnated with 1 ml of acetone solution. After the soluent evaporated, 10 ml of distilled water were added to form an inhibitor concentration of 100 yM. Seeds were planted directly on the moist papers and germinated for 4 days in a controlled environment chamber on a 16-hr photoperiod with 27+lC day temperature and 21+lC night temperature. Light intensity from both fluorescent and incandescent bulbs was 28 klux at dish level. Lipids were extracted and recovered from 1 g of lyophilized shoot tissue, separated into membrane and non-membrane lipids, and analyzed by gas chromatography as described (1). 

The Ar-(3-cyclopropyl-2,4-pyrimidyl)- 96a and hT-(3-cydopropyl-2,4,6-tri-azinyl)-AT-aryl sulfonyl ureas 96b (R = halo-, amino-, alkoxyalkyl and cycloalkyl X = CH, N) are useful as plant growth regulators and herbicides. At 500 g per ha the cyclopropyltriazine derivative 96 b (R = OMe, Ar = 2-carbomethoxy-phenyl) killed Abutilon species and Sivapis alba or prevented germination without affecting wheat, Eq. (37) [128]. 

In soil that has not had recent additions of plant residue or other organic material, microbial respiration proceeds at a low rate (13). Moreover, fungi apparently exist mostly as spores in a state of fungistasis. This microflora usually responds to the addition of plant residue by spore germination, increased respiration, and growth. These responses were induced by volatile components from alfalfa tops, corn leaves, wheat straw, bluegrass clippings, tea leaves, and tobacco leaves, even when the residue... 

Bioassav of Organic Compounds from the Soil. Bioassay experiments measured the germination and early growth (generally the most sensitive time of any plant s life) of wheat. The methods are similar to those of McPherson and Muller (151 and others in the field, and are summarized below. 

---