Plants ascorbate peroxidase

Yan J. Wang J. Tissue D. Holaday A. S. Allen R. Zhang H. (2003) Protection of photosynthesis and seed production under water-deficit conditions in transgenic tobacco plants that over-express Arabidopsis ascorbate peroxidase // Grop Sci. V. 43. P. 1477-483. 

II. Ascorbate Peroxidase (EC 1.11.1.11) APX isoenzyme, plays an important role in the metabolism of H202 in higher plants. APX utilizes Ascorbate (Asx) as its specific electron donor to reduce H202 to water with the generation of monodehydroascorbate, involved in the Ascorbate-GSH cycle. Thus, APX in combination with the effective Asx-GSH cycle functions to prevent the accumulation of toxic levels of H202. 

In plants, ascorbate is required as a substrate for the enzyme ascorbate peroxidase, which converts H202 to water. The peroxide is generated from the 02 produced in photosynthesis, an unavoidable consequence of generating 02 in a compartment laden with powerful oxidation-reduction systems (Chapter 19). Ascorbate is a also a precursor of oxalate and tartrate in plants, and is involved in the hydroxylation of Pro residues in cell wall proteins called extensins. Ascorbate is found in all subcellular compartments of plants, at concentrations of 2 to 25 mM—which is why plants are such good sources of vitamin C. 

Value at initial stage of culture Average APx activity in plant materials Amount of Chi on culture volume basis Average GPx activity in plant materials Average POD activity in plant materials Ascorbate peroxidase... 

Ascorbate peroxidase is a heme-eontaining enzyme that catalyses the ascorbate-dependent reduction of potentially damaging hydrogen peroxide in plants and algae, Eq. (1),... 

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