Pipetting aids

Pipet bulbs, or battery-operated pipetting devices—e.g., Pipet-Aid (Drummond Scientific)... 

Pipetting. All liquid transfer operations are performed with mechanical pipetting aids. Figure 3 also see the Source table (6). Oral pipetting is not performed in a laboratory engaged in research work with chemical carcinogens, suspect carcinogens, or other toxic substances (7). 

Spectroline Pipette Filler 26 Nalgene Pipetting Aid Outfit... 

Oxford Macro Set Transfer Pipetting System 41 Nalgene Pipetting Aid... 

Safety pipetting aids Mouth pipeting is prohibited it is a potential source of infection either by ingestion or by inhalation. All safety pipetting aids control contamination of the suction end of the pipet and leakage from the pipet tips. Vacuum lines used to aspirate liquids are protected with liquid disinfectant traps and cartridge-type filters (0.45 pm pore size). 

When pouring off the supernatant of the polystyrene microspheres and Concanavalin A, it is best to use a pipet aid to gendy siphon the supernatant off, as the pellet of microspheres is very easily disturbed. 

When the agar has cooled down, add the albumen to the agar solution and swirl gently to mix add 2.4ml of the lOOx antibiotic-antimycotic stock while continuing to mix. Using a sterile 10 ml pipet and pipet aid, distribute 2.5 ml agar-albumen into each petri dish, avoiding the formation of bubbles. Do not dispense more than 2.5 ml, since a thicker substrate will make observation of the embryos more difficult. 

Additional hazards of exposure to aerosols are created when liquid drops from a pipet to a work surface (474), when cultures are mixed by alternate suction and expulsion (209, 350, 499), when an inoculum is forcefully ejected onto a culture dish, or when the last drop is blown out (113, 209). It has been demonstrated by high-speed photography that an aerosol of approximately 15,000 droplets, most under ten micrometers in diameter, is produced when the last drop of fluid in the tip of a pipet is blown out with moderate force (Figure 1.1). While the aerosol hazard associated with pipetting procedures can only be reduced by use of safe techniques and use of biological safety cabinets (240), the potential hazards associated with oral ingestion can be eliminated by use of mechanical pipetting aids (426, 428). 

A list of safe pipetting techniques is shown in Table 1.1. Figures 1.2, 1.3, and 1.4 illustrate various pipetting aids. Many more varieties of... 

Never pipet by mouth. Always use some type 8. of pipetting aid. 

Figure 1.9 Handling glassware. The proper way to insert a pipet into pipetting aid Keep the hand close to the upper end of the pipet, and use a twisting motion of the pipet aid to insert the pipet. Do not force the pipet, or apply any amount of lateral pressure. (Source National Institutes of Health.)...

Mouth pipetting is not allowed. Appropriate pipetting aids must be used. 

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