Pipetting safety

Mechanical pipet fillers (sometimes called safety pipet fillers, propipets, or pi-fillers) are more convenient than latex bulbs (Figure 1.4C,D). Equipped with a system of hand-operated valves, these fillers can be used for the complete transfer of a liquid. The use of a safety pipet filler is oudined in Figure 1.5. Never allow any solvent or solution to enter the pipet bulb. To avoid this, two things must be kept in mind (1) always maintain careful control while using valve S to fill the pipet, and (2) never use valve S unless the pipet tip is... 

How to use a Spectroline safety pipet filler. Courtesy of Spectronics Corporation,West-bury, NY 11590. 

A 2-ml. aliquot of the solution, withdrawn by means of a safety pipet, is added to 20 ml. of water containing 2 g. of potassium iodide, and the liberated iodine is titrated with good shaking with standard sodium thiosulfate solution. 

Manostat Vari-Pet 25 Demuth Safety Pipet 30 Cornwall Continuous Pipetting... 

Safety pipetting aids Mouth pipeting is prohibited it is a potential source of infection either by ingestion or by inhalation. All safety pipetting aids control contamination of the suction end of the pipet and leakage from the pipet tips. Vacuum lines used to aspirate liquids are protected with liquid disinfectant traps and cartridge-type filters (0.45 pm pore size). 

Fancier safety pipet fillers such as the one illustrated in FIGURE B.6 may be available for your use in place of a rubber bulb. Safety pipet... 

Peleusball (Pipettierball) safety pipet filler, safety pipet ball Peptidbindung peptide bond, peptide linkage Peptonwasser peptone water Perameisensaure performic acid... 

Substances which destroy living tissues are described as corrosive and should never be handled without protective gloves and goggles. Concentrated sulphuric and nitric acids are known examples. The effect increases if the substance is hot. Safety pipetting is always essential especially in this case as well as odier precautions when dealing with toxic, harmful materials. 

In general, solid solutes should be weighed on weighing paper or plastic weighing boats, using an analytical or top-loading balance. Liquids are more conveniently dispensed by volumetric techniques however, this assumes that the density is known. If a small amount of a liquid is to be weighed, it should be added to a tared flask by means of a disposable Pasteur pipet with a latex bulb. The hazardous properties of all materials should be known before use and the proper safety precautions obeyed. 

Information on automatic pipets, procedures for use, and helpful hints. http //ehs.clemson.edu/bsm-spil.html Biological Safety Manual. http //www.hendrix.edu/chemistry/chemsafe.htm... 

M distilled water U pipets with different tip sizes H test tubes or small vials IS pH test strips Si small spatula IS crystallizing dish or beaker M chemical safety goggles M chemically resistant rubber gloves... 

Chemical Hazards. Chemical hazards are many and varied. It should be taken for granted that any chemical substance taken by mouth or inhaled is toxic until and unless definite assurance has been given to the contrary. Reactions that produce toxic fumes or vapors or entail risk of fire should always take place in a fume hood. As a matter of standard safety practice, never pipette any liquid or solution by mouth use a rubber pipetting bulb. Another insidious hazard is that of vapors from organic solvents. Such solvents should not be used indiscriminately for cleaning purposes, and spills should be avoided. Good ventilation is important. 

Biological (microbiological) safety cabinets In addition to accidental spillages, numerous laboratory procedures—pipeting, mixing, homogenizing, ultrasonic... 

Resuspend the crude RNA pellet in 25 pL of Buffer I. Use a Geiger counter to check that all of the labeled RNA has dissolved in solution by pulling up all the liquid into a pipet tip and comparing the counts in the tip with the counts remaining in the tube. Store in an appropriately shielded radioactive safety container at -80°C. 

---