Photobacterium damsela

Photobacterium damsela (x2,6-siaiyltransferase (Pd2,6ST) was the first bacterial sialyltransferase which has been cloned and purified by the Yamamoto group (33, 34). This enzyme has a relaxed acceptor specificity (35, 36). For example, it has been applied for the enzymatic sialylation of Tn glycopeptides with GalNAc a-linked to either serine or threonine residue) (37). It was also shown to be able to transfer sialic acid to both N- and 0-linkM glycoproteins (38). 

HANIF A, BAKOPOULOS V, LEONARDOS I and DiMiTRiADis G J (2005) The effect of sea bream Sparus auratd) broodstock and larval vaccination on the susceptibility by Photobacterium damsela subsp piscicida and on the humoral immune parameters. Fish Shellfish Immunology, 19, 345-361. 

NAGANO I, OSHIMA s and KAWAi K (2011) In vivo analysis on the adherence and infection route of Photobacterium damselae subsp piscicida in YellowtaU. Fish Pathology, 46,45-50. 

Yamamoto T, Nakashizuka M, Terada I. Cloning and expression of a marine bacterial beta-galactoside 2,6-sialyltransferase gene from Photobacterium damsela JT0160. 7. Biochem. 1998 123 94-100. 

Example of the one-pot, three-enzyme synthesis of Siaa2-6GalbpNP (32) and Siaa2-3GalppNP (33). Abbreviations Pm NeuA aldolase from, Pasteurella multocida NmCSS CMP-synthetase CMP-sialic acid synthetase from Neisseria meningitidis PmSTI P. multocida sialyltransferase Pd2,6ST a2-6-sialyltransferase from Photobacterium damsela GaippNP p-nitrophenyl p-galactoside. 

DOS SANTOS NMS, TAVERNE-THIELE JJ, BARNES AC, VAN MUISWINKEL WB, ELLIS AE, ROMBOUT JHWM (2001), The gill is a major organ for antibody secreting cell production following direct immersion of sea bass (Dicentrarchus labrax, L.) in a Photobacterium damselae ssp.piscicida bacterin an ontogenetic study , Tw/z Shellfish Immunol, 11, 65-74. 

Influence of vaccination on the nitric oxide response of gilthead seabream following infection with Photobacterium damselae subsp. piscicida. Fish Shellfish Immunology 18,31-38. 

BAKOPOULOS, V., PEARSON, M., VOLPATTI, D., GOUSMANI, L., ADAMS, A., GALEOTTI, M. and DiMiTRiADis, G.j. (2003) Investigation of media formulations promoting differential antigen expression by Photobacterium damsela ssp. piscicida and recognition by sea bass, Dicentrarchus labrax (L.), immune Journal of Fish Diseases 26, 1-13. 

NiTZAN, s., SHWARTSBURD, B. and HELLER, E.D. (2004) The effect of growth medium salinity of Photobacterium damselae subsp. piscicida on the immune response of... 

THUNE, R.L., FERNANDEZ, D.H., HAWKE, J.P. and MILLER, R. (2003) Constmction of a safe, stable, efficacions vaccine against Photobacterium damselae ssp. piscicida. Diseases of Aquatic Organisms SI, 51—58. 

Pasteurellosis, caused by Pasteurella piscicida, also named Photobacterium damsela subsp. piscicida is one of the major diseases in many species of wild and farmed fish in Asia, USA and Europe. The pathogen is causing disease in yellowtail (Seriola quinqueradiatd) aquaculture in Japan (Kawakami et al. 1998). Vaccination with an LPS-mixed chloroform-killed bacterin resulted in protection against challenge with the virulent bacterium. Inclusion of FCA in the vaccine did not significantly enhance the protective effect (Kawakami et al. 1998). 

---