Phosphokinases

As an activator of the phosphokinases, magnesium is essential in energy-requiring biological processes, such as activation of amino acids, acetate, and succinate synthesis of proteins, fats, coen2ymes, and nucleic acids generation and transmission of nerve impulses and muscle contraction (67). 

Fig. 4. Requirements, substrates, and products of Mo-nitrogenase catalysis, where I is the MoFe protein II the Fe protein and Pi is inorganic phosphate. The generating system is composed of creatine phosphate and creatine phosphokinase to recycle the inhibitory MgADP produced during catalysis to...

C2 domains (phosphokinase C conserved 2 domains) mediate membrane targeting of diverse peripheral proteins. A C2 domain consists of approximately 130 residues and was first discovered as the Ca2+-binding site in conventional phosphokinase Cs. 

Takahashi, R. Ushikubo, S. Oimomi, M. and Shinki, T. Creatine phosphokinase isoenzymes of human heart muscle and skeletal muscle. Clin. Chim. Acta (1972),... 

CK Creatine phosphokinase CKMB The myocardial-specific isoenzyme of creatine phosphokinase Cl The chemical symbol for chloride CL Chemiluminescent CLA Cutaneous lymphocyte antigen CL18/6 Anti-ICAM-1 monoclonal antibody... 

Succinylcholine 1-1.5 mg/kg IV up to 150 mg total dose ° Contraindications may include use in patients with a personal or family history of malignant hyperthermia, extensive/severe burns, myopathies with elevated creatine phosphokinase, penetrating eye injuries, pre-existing hyperkalemia, narrow-angle glaucoma, and disorders of plasma pseudocholinesterase... 

Check periodic creatine phosphokinase levels with prolonged infusions (especially if patient is receiving concomitant corticosteroid pharmacotherapy)... 

MM An isoenzyme of creatine phosphokinase OU Oculus uterque (each eye)... 

Creatine Phosphokinase (CPK) (rabbit skeletal muscle Calbiochem) Resuspended with deionized RNAse free water to a final concentration of 14 mg/ml and stored at —20°. 

CPK creatine phosphokinase FRAP fluorescence recovery after photolysis... 

Daily gavage of 50, 500, or 2500 pg/kg BW for 102 weeks Dose-related mortality in males during first year and in females during entire study significant lethality in the 500 and 2500 pg/kg groups. No increased incidence of microscopic neoplastic or nonneoplastic lesions in treated rats decreased creatinine phosphokinase levels in treated rats 28... 

After 48 h, marked increase in blood glucose, depressed plasma insulin level, marked depletion of liver glycogen, significant increase in plasma creatinine phosphokinase and glutamic oxaloacetic transaminase activity (Giri etal. 1979)... 

Physical symptoms include dizziness, dysarthria, ataxia, nystagmus, lid ptosis, tachycardia, sweating, and increased deep tendon reflexes. Most subjects show some degree of hypertension, associated with increased minute and tidal volumes of respiration, increased formation of urine, and increased muscle tone. The latter may lead to increased serum creatinine phosphokinase concentration. With very large doses, convulsions and respiratory arrest are the terminal events (11). The course of clinical symptoms and signs following various doses of PCP is shown in Table 2. 

Five repeated exposures of 200 ppm for 12.5 min every 4 d resulted in increased cardiac-specific creatine phosphokinase activity in the blood (pooled data measured at 2 h after the first, third, and fifth exposures) and ectopic heart beats during the first 2 min after injection of norepinephrine (after the fifth exposure) but failed to induce cardiac lesions (histopathologic examinations at 14 d postexposure) (O Flaherty and Thomas 1982). The rats were restrained and anesthetized. 

Sidell, F.R., Calver, D.L. and Kaminskis, A. (1974). Serum creatine phosphokinase activity after intramuscular injection. JAMA 228 1884-1887. 

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