Phosphate isolation

It has recently been reported21 that a mixed osazone of 3,4-di-O-methyl-D-glucose can be converted, by treatment with p-nitrobenzaldehyde, into an osone which reacts with phenylhydrazine to give 3,4-di-O-methyl-D-glucose phenylosazone. Von Lebedev29 claimed to have obtained D-glu-cosone 6-phosphate, isolated as an amorphous lead salt, by the action of hydrochloric acid on the phenylosazone prepared from D-fructose 6-phosphate. 

Fig. 12.—Chemical structure of an O-acetylated AUV -bis(3-acetoxytetradecanoyl) tetrasac-charide tetramethyl phosphate isolated from the E. coli Re mutant FS1S (34). [For details see text.]...

The crude product was deacetylated with methanolic ammonia and the unprotected / -phosphate isolated as the monoammonium salt by column chromatography on silica gel. 

Gagnaire, V., Pierre, A., Molle, D., and Leonil, J. 1996. Phosphopeptides interacting with colloidal calcium phosphate isolated by tryptic hydrolysis of bovine casein micelles. J. Dairy Res. 63, 405-422. 

Kalckar117118119 has shown that the enzymatic phosphorolysis of inosine (hypoxanthine 9-D-ribofuranoside) may give rise to the formation of a pentose phosphate, isolable as its barium salt. The phosphate was found to be non-reducing although easily hydrolyzed by either acid or alkali to equimolar quantities of phosphate and pentose. In view of these properties and the fact that it could be used for the enzymatic synthesis of purine ribosides, Kalckar has tentatively assigned to it the D-ribose 1-phosphate structure its ring structure and configuration at carbon 1 remain undetermined. 

While these results support a phosphate ester bridge between the glycerol and inositol, it does not prove where the bond is located on the inositol molecule. Inasmuch as the inositol phosphate isolated by alkaline cleavage from the parent phosphatidylinositol or the glycerophosphoinositol was optically active, the phosphate must be attached at the 1 or 4 position. Substitution at the 2 position of inositol would not yield an optically active product. Subsequently as will be discussed, observations from several laboratories have shown that the phosphate bond is between the sn- 3 position of the glycerol and the C-l hydroxyl on myo-inositol. 

Table 12-2 Analysis of Glucose-1-Phosphate Isolated from Potato Phosphorylase Reaction pmol of Reducing /xmol of Inorganic Equivalents/0.1 ml Phosphate/0.1 ml...

Scholz, S., Sonnenbichler, J., Schafer, W., and Hensel, R., 1992, Di-myo-inositol-1,1 -phosphate A new inositol phosphate isolated from Pyrococcus woesei. FEBS Lett. 306 239-242. 

Analysis of the enzymically synthesized product by chemical and enzymic methods, as well as by optical rotation, showed that it was identical with the trehalose phosphate isolated from the fermentation products of dry yeast. ... 

Sugar phosphates are present in soils (Anderson and Malcolm, 1974 Sanyal and De Datta, 1991). Some organic phosphates isolated from organic matter display the properties characteristic of phosphopro-teins (Anderson, 1961). Several monophos-phorylated carboxylic acids have also been detected in soil extracts (Anderson and Malcolm, 1974). Much organic phosphorus exists in highly combined forms and remains unidentified. 

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