Phenotypic high-content screening

The siRNA screening is performed in duplicate in 96-well plates as shown in Fig. 1. The first replica is used for viability readout and the second for phenotypic readout where we look at spindle structure by using a high content screening platform. The small molecule library screening is also performed in duplicate with the first replica used for viability readout. In contrast to the siRNA... 

Smaller compound sets and focused compound libraries can be used to screen more broadly for physiological phenotypes. In the typical high-content screening experiment, the effects of compounds on cellular assays are captured... 

Section 5.5 Targets Arising from Phenotype or High-Content Screening... 

High-content screening (HCS) is a cell assay method in which phenotypic changes in live cells... 

TABLE 8.1 Comparison of Outcomes between High Content Screening (HCS) Using HepG2 (Johnson Johnson), Human Hepatocytes (Pfizer), and Zebrafish Phenotypic Hepatotoxicity Screening (Evotec) against FDA Liver Toxicity Knowledge Base (LTKB) DILI Classification... 

High-content screen (HCS) is a phenotypic screening that monitors multiple cellular parameters simultaneously. HCS employs fluorescence-based reagents (antibodies, dyes that bind or localize to a given cell component, sensors) to generate a multicolor fluorescence readout that is usually recorded using automated optical image acquisition devices. 

Key words Zebrafish, Small molecules. Phenotypic screening. High content screening. Cardiovascular... 

This screening protocol uses qualitative measures as a read out. However, many high content screening (HCS) tools have been developed which are capable of imaging zebrafish in 96-well formats and could result in more quantitative measures for the phenotype screened. 

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