Phaseolus vulgaris composition

Bennett, M.J. Cullimore, J.V. (1989). Glutamine synthetase isoenzymes of Phaseolus vulgaris L. subunit composition in developing root nodules and plumules. Planta 179, 433-40. 

Cai, X. Wong, P.P. (1989). Subunit composition of glutamine synthetase isoenzymes from root nodules of bean (Phaseolus vulgaris L.). Plant Physiology 91, 1056-62. 

The trypsin inhibitors from several different varieties of Phaseolus vulgaris (common bean) are quite variable in amino acid composition (Figure 5). ASN, SER and 1/2CYS are found in the largest amounts in most of the inhibitors. MET and TRP are quite low in all the inhibitors and absent in a substantial number of them. PRO is present in larger amounts than in most proteins. 

Multiple forms of cysteine synthase have been demonstrated in a number of plants. Two peaks of cysteine synthase were obtained by ammonium sulfate fractionation of acetone powders of spinach (Giovanelli and Mudd, 1968). A highly purified cysteine synthase from rape leaves was resolved into two fractions by polyacrylamide gel electrophoresis. The two had similar sizes and subunit compositions but one contained pyridoxal phosphate, whereas the other contained a derivative of pyridoxal phosphate (Masada et al., 1975). The electrophoretic mobility of the latter enzyme could be converted to that of the former by incubation with pyridoxal phosphate. Two forms of cysteine synthase have been extracted from both Phaseolus vulgaris and P. polyanthus (Table II) (BertagnoIIi and Wedding, 1977). It is unlikely that the multiple forms of the Phaseolus enzyme are due to modification of the pyridoxal phosphate prosthetic group, since pyridoxal phosphate was included in all media used for purification and assay of these forms. 

Zacharius, R. M. Composition of the nitrogenous components of the bush bean seed (Phaseolus vulgaris) including isolation of 5-acetylornithine. Phytochemistry 9, 2047 (1970). 

Two groups of isolectins have been isolated from Phaseolus vulgaris seeds after chromatography on immobilized fetuin. Although the lectins in the two groups have similar amino-acid and sugar compositions, they share only a partial structural identity as demonstrated by immunochemical criteria. 

The composition of the neutral sugars comprising the pectins of Phaseolus vulgaris has been shown to fluctuate during the culture cycle of suspensions of cotyledon cells, whereas only small changes were observed in the monosaccharide composition of the neutral polysaccharides. The pectin from the bark of the white willow Salix alba has been partially depolymerized with acid. Neutral disaccharides [(16) and (17)] and acidic oligosaccharides [(18)—(20)] isolated from the hydrolysates were characterized by g.l.c.-m.s. of their methylated derivatives. 

Benveniste, Isolement, caracterisation et composition en sterols de fractions subcellulaires de feuilles etiolees de Haricot. Physiol. Veg. 11 209 (1973). b) R.D. Brandt and P. Benveniste, Isolation and Identification of sterols from subcellular fractions of bean leaves (Phaseolus vulgaris). Biochim. Biophys. Acta 282 ... 

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