Phage, recombination system

Gateway cloning technology is a modification of the recombination system of phage k (Walhout et ah, 2000 Hartley et ah, 2000). The Gateway system utilizes a minimum set of components of the k system for in vitro transfer of DNA, namely the k integrase protein, k excisionase, the E. coli protein... 

The first site-specific recombination system studied in vitro was that encoded by bacteriophage A. When A phage DNA enters an E. coli cell, a complex series of regulatory events commits the DNA to one of two fates. 

The third method of joining heavy and light chains involves the use of recombination systems. One phage library in the Fab format has been constructed in this fashion [4], Separate VH+CH1 and VL+CL libraries were made in different vectors. The VH+CH1 library was cloned into a plasmid vector with the VH+CHI domains flanked by two recombination signals... 

Deng X K, Nesbit L A, Morrow K J, Jr (2003). Recombinant single-chain variable fragment antibodies directed against Clostridium difficile toxin B produced by use of an optimized phage display system. Clin. Diagn. Lab. Immunol. 10 587-595. 

Three loci of the recombination system, two loci of integration, one locus of bacterial metabolic suppression, two loci of duplication, and the Cn and Cm loci which control the selection of developmental pathways (lytic growth vs lysogeny) begin to function within the first 5-7 min. Transcription of the late loci is possible only after circularization of the phage DNA. This conversion of DNA structure, as mentioned above, occurs very early and requires the aid of bacterial ligase. 

T4 DNA Pol has been prepared traditionally from the T4 phage-infected E. colt B which contains 600 polymerase molecules per cell. T4 DNA Pol can now be more efficiently prepared from recombinant systems in which gene 43 has... 

There are currently 18 monoclonal antibodies which have been licensed for therapeutic use and this therapeutic category is expanding rapidly. The majority of these antibodies have been produced by recombinant DNA technology such as the phage display system but there are three which are produced by the more old-fashioned murine antibody technology produced in hybridomas. There is currently quite a number of Fab fragments in clinical trials. 

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