Phage cDNA expression libraries

Figure 10.22 Screening of the frog cDNA phage-expressed library Lll to find macromolecu-lar binding partners for SH3 protein domains the selection/amplification process using the biotinylated selection substrate 10.18.

R. A. Williamson G. J. Silverman, Gene Fragment Libraries and Genomic and cDNA Expression Cloning. In Phage Display A Laboratory Manual, C. F. Barbas, III, D. R. Burton, J. K. Scott, G. J. Silverman, Eds. Cold Spring Harbor Laboratory Press New York, 2001 p 6.1. 

One type of expression library is created by insertion of the cDNAs into copies of a bacteriophage gene that permits the expressed proteins to be displayed on surfaces of the phages (see Fig. 3-16). 

Crameri, R., Jaussi, R., Menz, G., and Blaser, K. (1994). Display of expression products of cDNA libraries on phage surfaces. Eur. J. Biochem. 226, 53-58. 

Peptide libraries Intermediaries for non peptide drugs Mirror image phage display Peptides with effector functions Select binders on unknown antigens From 2D gels From expression cDNA libraries... 

To circumvent some of the limitations of direct immunization, phage display technology has been applied to the preparation of fully human MABs. Gene libraries of cDNA from nonimmune or immunized donor lymphocytes are expressed in bacteriophages. The bacteriophages display functional antibody fragments and can... 

To identify relevant IgE binding components from natural source extracts, various techniques like IgE immunoscreening of expression cDNA libraries, reverse transcriptase polymerase chain reaction (PCR), or phage-display technology combined with immunoscreening are used (Wallner et al. 2004). 

Crameri R, Elemmann S, Blaser K, pJuFo A phagemid for display of cDNA libraries on phage surface suitable for selective isolation of clones expressing allergens, Adv. Exp. Med. Biol., 409 103-110, 1996. 

Jaspers LS, De Keyser A, Stanssens PE, Lambda ZLG6 A phage lambda vector for high-efficiency cloning and surface expression of cDNA libraries on filamentous phage, Gene, 173 179-181, 1996. 

Crameri R, Jaussi R, Menz G, Blaser K Display of expression products of cDNA libraries on phage surfaces. A versatile screening system for selective isolation of genes by specific gene-product/ligand interaction. Eur J Biochem 1994 226 53-58. 

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