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Peroxidase antiperoxidase complex

Moriarty GC, Moriarty CM, Sternberger LA (1973) Ultrastructural immunocytochemistry with unlabelled antibodies and the peroxidase-antiperoxidase complex. A technique more sensitive than radioimmunoassay. J Histochem Cytochem 21 825-836... [Pg.30]

Bronckers, A.J.J., Gay, S., Finkelman, R.D., and Butler, W.T. (1987) Immunolocalization of Gla proteins (osteocalcin) in rat tooth germs Comparison between indirect immunofluorescence, peroxidase-antiperoxidase, avidin-biotin-peroxidase complex, and avidin-biotin-gold complex with silver enhancement./. Histochem. Cytochem. 35, 825-830. [Pg.1051]

S.M. Hsu, L. Raine, and H. Fanger, A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am. J. Clin. Pathol 75, 734-738 (1981). [Pg.399]

Swanson, P., Hagen, K., and Wick, M. (1987) peroxidase-antiperoxidase (ABPAP) Avidin-biotin-complex. Am. J. Clin. Pathol. 88, 162-176. [Pg.190]

Elias, J., Margiotta, M., and Gabore, D. (1989) Sensitivity and detection efficiency of the peroxidase antiperoxidase (PAP), avidin-biotin complex (ABC), and the peroxidase-labeled avidin-biotin (LAB) methods. Am. J. Clin. Pathol. 92,62. [Pg.214]

The ABC detection system has been shown to be more sensitive than most other detection system (5,6), primarily because of the large size of the preformed ABC complexes, which result in amplification of the signals. Alternative detection systems for immunohistochemical analysis include the peroxidase-antiperoxidase (PAP) (1) and the alkaline phosphatase-antialkaline phosphatase (APAAP) systems (7) (see Chapter 24). These approaches are conceptually and technically similar, and will not be discussed here. [Pg.216]

The avidin-biotin complex (ABC) method and the streptavidin-biotin (SAB) method are more sensitive than the peroxidase-antiperoxidase (PAP) method for histochemical techniques. The strong noncovalent attraction between biotin and avidin or streptavidin is exploited in many histochemical, immunohistochemical, and in situ hybridization... [Pg.98]

The sections are incubated for 72 hr at 4°C with primary antiserum in PBS containing 2% normal serum and 0.25% gum arabic (Sigma). The sections are then incubated overnight at 4°C with a biotinylated secondary antibody (Vector Laboratories, Burlingame, CA), diluted 1 200. They are next incubated overnight at4°C with avidin-biotinylated peroxidase complex (ABC) diluted 1 100 in PBS containing 0.25% gum arabic. Note that the peroxidase-antiperoxidase procedure can also be used. [Pg.178]

An immunohistochemical examination of PSA using polyclonal antibodies by the peroxidase antiperoxidase (PAP) method and by the technique of biotin-streptavidin-alkaline phosphatase has been successfully carried out (Zaviacic et al., 1994). Immunoelectron microscopy in conjunction with the protein A-gold complex can also be used for localizing PSA in human prostate (Sinha et al., 1987). The procedure for immunofluorescence localization of PSA is given below. [Pg.203]

Noncovalent binding of HRP to antibody, also known as unlabeled antibody binding, is described in great detail by Sternberger (1). Instead of the use of bifunctional reagents, IgG class antibodies to HRP are used to form a soluble semicyclic immune complex consisting of two antibody and three enzyme molecules. The molecular weight of the peroxidase antiperoxidase, PAP complex is 400 - 430 kDa. [Pg.16]

PO. .. peroxidase, PAP... antiperoxidase complex (Reproduced by kind pomission of Van Nostrand Reinhold Company)... [Pg.210]

Sequential localization of two antigens in the same specimen by peroxidase-antiperoxidase (PAP) complexes without intermittent antibody removal... [Pg.468]

Sternberger LA, Sternberger NH (1986) The unlabeled antibody method comparison of peroxidase-antiperoxidase with avidin-biotin complex by a new method of quantification. J Histochem Cytochem 34 599-605... [Pg.11]

Swanson P, Hagen K, Wick M (1987) Avidin-biotin-peroxidase-antiperoxidase (ABPAP) complex. Am J Clin Path 88 162-176... [Pg.241]

Peroxidase antiperoxidase (PAP) complex e.g rabbit PAP complex raised in the same species as primary antiserum (DAKO). [Pg.78]

Fig. 1. Diagram illustrating the molecular interactions of the PAP procedure. The PAP complex is comprised of horseradish peroxidase bound to an antiperoxidase antibody generated in the same animal species as the primary antibody which recognized the tissue antigen of interest. The primary antibody and the PAP complex are linked via a secondary antibody generated in a second animal species against immunoglobulin of the primary animal species (A, immunoglobulin , peroxidase enzyme). Fig. 1. Diagram illustrating the molecular interactions of the PAP procedure. The PAP complex is comprised of horseradish peroxidase bound to an antiperoxidase antibody generated in the same animal species as the primary antibody which recognized the tissue antigen of interest. The primary antibody and the PAP complex are linked via a secondary antibody generated in a second animal species against immunoglobulin of the primary animal species (A, immunoglobulin , peroxidase enzyme).
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