Avidin biotinylated

Fig. 1. Diagram illustrating the molecular interactions of the ABC procedure. The primary antibody against the antigen of interest is linked to the avidin-biotinylated peroxidase complex via a biotinylated secondary antibody raised against immunoglobulin of the animal species used to generated the primary antibody (A, immunoglobulin , biotin A, avidin , peroxidase).

As conjugates, complexes from biotinylated hapten and strep-tavidin maybe used. In that case perform the detection with avidin-biotinylated hapten because avidin and streptavidin do not cross-react. 

The sections are incubated for 72 hr at 4°C with primary antiserum in PBS containing 2% normal serum and 0.25% gum arabic (Sigma). The sections are then incubated overnight at 4°C with a biotinylated secondary antibody (Vector Laboratories, Burlingame, CA), diluted 1 200. They are next incubated overnight at4°C with avidin-biotinylated peroxidase complex (ABC) diluted 1 100 in PBS containing 0.25% gum arabic. Note that the peroxidase-antiperoxidase procedure can also be used. 

O Silver Island Films HRP-labeled Avidin Biotinylated BSA... 

Fig. 2. Distribution of glutaminase-like immunoreactivity in the central nervous system. The rat brains were fixed and immunostained as reported before (Kaneko et al., 1989). Briefly, rats were fixed at room temperature by transcardial perfusion of 0.2% formaldehyde, 75%-saturated picric acid and 0.1 M sodium-phosphate, pH 7.0, and the brain blocks were further placed for 3 days at 4°C in 2% formaldehyde, 75%-saturated picric acid and 0.1 M sodium-phosphate, pH 7.0. The brain sections (30 p.m thick) were incubated with 10 p.g/ml monoclonal anti-glutaminase IgM, MAb-120, then with 10 p.g/ml biotinylated goat anti-glutaminase antibody (Vector), and finally with avidin-biotinylated peroxidase complex (ABC Vector), and the bound peroxidase was developed brown by reaction for 10-30 min with 0.02% diaminobenzidine-4HCl (DAB), 0.003% H2O2 and 50 mM Tris-HCl, pH 7.6. (o) Upper cervical cord.

Avidin/Biotinylated Enzyme Complex (ABC) kit (Vector Laboratories, Burlingame, CA). 

Glucose oxidase polypyrrole biotinylated polypyrrole / avidin/biotinylated glucose oxidase amperometric 49... 

Figure 15 (A) Faradaic impedance spectra of (a) The (17)-functionalized An electrode, (b) After interaction of the sensing electrode with (18) (5 X 10" M), which was pretreated with (20) (1 X 10 M, 30 min, 25°C). (c) After treatment of the resulting electrode with avidin (2.5 pg-mL" ). (d) After interaction with the biotinylated liposomes, (21). (e) After treatment of the interface for a second time with avidin (2.5 0,g mL" ), (f) After interaction of the interface for a second time with the biotinylated liposomes, (21). Data were recorded in 0.1 M phosphate buffer, pH = 7.2, in the presence of [Fe(CN)g] " , (1 1), as redox-probe. (B) Calibration curve corresponding to the changes in the electron transfer resistances of the sensing electrode upon interaction with the analyte DNA, (18), at different concentrations and enhancement of the sensing process by a double-step avidin/ biotinylated liposome amplification path. corresponds to the difference in the electron transfer resistance after a double-step avidin/biotinylated liposome amplification and the electron resistance of the (17)-functionalized electrode.

Signal amplification use preformed avidin/biotinylated enzyme complex (e.g., VECTASTAIN Elite ABC kit (Vector Laboratories, Inc., Burlingame, CA) (caution store at 4 °C). 

GOx biotinylated GOx Glucose Alkanethiols w-carboxyalkanethiols cysteamine ferrocenylalkanethiols aminoalkanethiols avidin-biotinylated dialkyl disulfide Au Amperometric 91-97... 

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