Peptone yeast medium

Kenny (1973) recommends a Soy peptone-yeast dialysate medium (Appendix 4) for culture of mycoplasma. Inclusion of arginine (16 mM) and 0.4 mg% phenol red indicates the presence of arginine deaminase by formation of alkali (purple coloration). Alternatively, incubation with tritiated thymidine and analysis of the culture medium for tritiated thymine can be used to detect thymidine phosphorylase (House and Waddell, 1967). 

First of all, we generally used a nutrient medium composed of peptone, yeast extracts, and glycerol when culturing several of the bacteria we had isolated. The yeast extract, namely its type and lot, was found to be a major factor so we needed to select the most stable variety (Fig. 14). 

Screening of Fungi for Alachlor Degradation. Fungal isolates CCF-1 (tentatively identified as Fusarium sp.) and CCF-2 degraded more than 70% and 50%, respectively of a 100 ppm dose of alachlor after 14 days of incubation in peptone-yeast extract medium (PYA1). About 18% of the... 

During Escherichia coli cultivation on casein peptone, yeast and meat extract as medium components and in presence of an antifoam agent, the surface tension (36mNm ) and surface viscosity (1.9 cP) were constant and did not depend on the cultivation time. 

The stated quantities of soya peptone, yeast extract, trypticase, potassium dihydrogen phosphate. Tween 80 and agar-agar are dissolved in the demineralized water by heating. The stated quantity of sodium carbonate is then added. As a result of the buffer effect of the sodium carbonate, it is not necessary to set the pH. The solution is sterilized by being heated in an autoclave for 20 minutes at 121 C. The culture medium is then cooled down to kG C. Then 10 ml of a 1 % sterile-filtered aqueous solution of TTC and ml of 10 % sterile-filtered aqueous solution of sodium azide solution are added. After mixing well, the culture medium is poured out into sterile Petri dishes. 

Cultural characteristics of Pectimtus species have been studied in peptone yeast extract fructose (PYF) medium (http //culturecollection.vtt.fi) and MRS medium. The beer-spoilage species form circular, entire, glistening and opaque colonies on PYF... 

Acetic acid is produced from complex basal medium (eg., peptone-yeast extract broth). 

COMPLEX BASAL MEDIUM CATABOLISM (E.G., PEPTONE YEAST EXTRACT BROTH)... 

Cadina-4,10(15)-dien-3-one (265) possessing insecticidal and ascaricidal activity, from Jamaican medicinal plant Hyptis verticillata was metabolized by Curvularia lunata. ATCC 12017 in potato dextrose to give its 12-hydoxy- (266), 3a-hydroxycadina-4,10(15)-dien (267), and 3a-hydroxy-4,5-dihydrocadinenes (268) while 265 was incubated by the same fungus in peptone, yeast, and beef extracts and glucose medium, only 267 and 268 were obtained. Compound 267 derived synthetically was treated in the same fungus Curvularia lunata to afford three metabolites (269-271) (Collins and Reese, 2002) (Figure 15.82). 

Nutrients The most well-known lactic acid producing organisms, such as Lactobacillus and Lactococcus species, are members of the taxonomic order of Lactobacil-lales, also commonly referred to as lactic acid bacteria. These lactic acid bacteria have their really complex nutrient need in common [29]. Vitamins and peptides need to be added to the medium to enable growth. This can be done by adding peptones, yeast extract, or corn steep liquor, but this is expensive. Nutrients for lactic acid production can also be derived from nutrient-rich waste streams such as rice bran, fish waste, or vinification lees [46 8]. 

Medium A formulation composed of various ingredients that will support the growth of different microorganisms. Such ingredients include glucose, peptone, yeast extract, liver extract, and others. A medium can be prepared as a liquid or solid, the latter with the addition of agar. 

Anaerobiospirillum succiniciproducens is a strict anaerobe and grows at an optimal temperature of 39 °C. A typical fermentation medium contains dextrose, peptone, yeast extract, and salts. The optimal pH range for this organism was determined to be between 5.8 and 6.4. This organism can produce approximately 30 g of succinate/1 fi-om a starting glucose concentration of 50 g/1. Calcium hydroxide is added to produce a calcium succinate product, which can be precipitated fi-om the broth (Datta 1992). 

Fig. 1. Effect of chloramphenicol on growth of B. hrevis Vm4 and production of edeine when added in early logarithmic phase. Inoculated with B, hrevis Vm4, Bacto-peptone-yeast extract medium was incubated on a rotary shaker at 32 C. Growth (x) control (o) in the presence of I0(xg/ml of chloramphenicol (arrow shows time of addition of chloramphenicol). Edeine production (A) control ( ) in the presence...

Two 300 ml Erlenmeyer flasks are prepared, each of them containing 60 ml of the following vegetative medium in tap water 0.6% peptone, 0.3% dry yeast and 0.05% calcium nitrate. The pH after sterilization by heating in an autoclave to 120°C for 20 minutes is 7.2. 

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