Casein peptone

Amino acids (Factor B, experiment 1) were supplied as casein peptone 35 g/L (Marcor) or casamino acids 25.8 g/L (Marcor). 

Example number 1. Medium for fermentation. The goal of this experiment was to develop a defined fermention medium that was usable for the manufacture of ImuVert The objectives were to evaluate the effect of replacing citrate with a phosphate buffer, yeast extract with vitamins and minerals, casamino acids with casein peptone and high or low O2 levels on the final yield and composition of ImuVert. 

Confirmation of the results obtained in the above experiments was obtained by completing two additional runs using yeast extract, caseine peptone, kosers citrate and... 

Figure 1. Half Effect Results for Yield (A) or Vesicle to Ribosome Ratio (B). A is the effect of vitamins and yeast extract. B is the effect of casein peptone and casamino acids. C is the effect of dissolved O2. D is the effect of citrate and phosphate. AB is the interaction of yeast extractAdtamin and mineral with casein peptone/casamino acids. AC is the interaction of yeast extractAdtamins and minerals with dissolved O2. BC is the interaction of casein peptone/casamino acids with dissolved O2. CD is the interaction effect of dissolved 2 with the citrate or phosphate.

Brandt, V., Teschemacher, H., Henschen, A., Lottspeich, F. Novel opioid peptides derived from casein (beta-casomorphins). I. Isolation from bovine casein peptone, Hoppe-Seyler s Z. Physiol. Chem. 1979, 360, 1211-1216. 

The original HL-5 medium was slightly modified in several laboratories. For example, HL-5C contains casein peptone and bactotryptone and a slightly different phosphate buffer composition [105]. In 1977, Franke and Kessin [106] published the development of a defined minimal medium... 

Fig. 2 Xylanase production in the multi-layer-packed bed reactor with passive aeration conditions a specific activity, b total activity. X represents 50 g of soya oil cake supplemented with 200 ml of the nutritive solution [casein peptone at 0.75% (w/v) and Na2HP04.2H20 at 1% (w/v) in distilled water]. Inoculum is 5.10 spores/gram of soya oil cake or the results of the development of these spores in 1 day (mycelia). The ratio values are averages based on addition of the enzymatic activity from the four layers, and the experiment was performed in triplicate. The deviation standard was below 8% in all cases. Conidial inoculum ...

The spectrophotometric evidence reviewed above for the binding of a proportion of the phenolic hydroxyl groups of the tyrosine residues of native proteins is supported by work on the action of tyrosinase on proteins. Sizer (1946) found that this enzyme oxidizes the tyrosine residues in native trypsin, pepsin, chymotrypsin, casein, peptone, insulin, and hemoglobin. Native ovalbumin, human and bovine serum albumin, tobacco mosaic virus (nucleoprotein), human y- and bovine /3-globulins, and bovine fibrinogen are not susceptible to tyrosinase, but become so after tryptic digestion. It was shown (Sizer, 1947) that for the proteins which are oxidized by tyrosinase in the native state, the observed reaction does indeed occur with the intact proteins and does not require preliminary degradation to tyrosine peptides or free tyrosine. The kinetics of the oxidation of tyrosine by tyrosinase have been studied spectropho-tometrically (Mason, 1948 etc.). 

During Escherichia coli cultivation on casein peptone, yeast and meat extract as medium components and in presence of an antifoam agent, the surface tension (36mNm ) and surface viscosity (1.9 cP) were constant and did not depend on the cultivation time. 

Escherichia coli casein peptone (small amount of Desmophen) 1.40... 

These methods can be used for the determination of tyrosine in serum albumin and some peptides, and all three amino acids in a hydrolysate of casein (pepton). 

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