Peptides detection systems

Thannhauser, T. W., McWherter, C. A., and Scheraga, H. A., Peptide mapping of bovine pancreatic ribonuclease A by reverse-phase high-performance liquid chromatography. II. A two-dimensional technique for determination of disulfide pairings using a continuous-flow disulfide detection system, Anal. Biochem., 149, 322, 1985. 

Recently, introduction of DNA analogous detection systems such as the use of peptide nucleic acid (PNA) technology has attracted considerable attention [10]. The PNAs are synthetic analogues of DNA that hybridise with complementary DNAs or RNAs with high affinity and specificity, essentially because of an uncharged and flexible polyamide backbone. The unique physico-chemical properties of PNAs have led to the development of a variety of research and diagnostic assays where these are used as molecular hybridisation probes [11]. 

Combinatorial approaches in synthetic chemistry allow the high-throughput screening of millions of compounds. It is the aim to establish such approaches in biosynthetic systems by creating module assemblies, which provide a new tool to create peptide libraries. The large number of different modules in peptide synthetase systems is suitable for creating a pool of many different enzymes in order to detect a useful compound in the near future [2,51],... 

Nishino, N., Makinose, Y., and Fujimoto, T. 1992. 2-Aminobenzoyl-peptide-2,4-dinitroanilinoethylamides facile fluorescent detection system for sequence specific proteases. Chem. Lett. 1, 77-80. 

Analytical determination of peptides in wine requires sample preparation, involving their isolation from the remaining components, mainly high molecular weight nitrogen compounds, free amino acids and phenols. Table 6B.1 summarizes the procedures used in the literature for the extraction of wine peptides before their analysis by different analytical techniques and with different detection systems. 

Takahashi, M. Nokihara, K. Mihara, H., Construction of a protein-detection system using a loop peptide library with a fluorescence label. Chem. Biol. 2003, 10, 53-60... 

Some properties of phosphopeptides make them preferable to the native phosphoprotein substrates for use with phosphate detection systems. The values of for peptide substrates are two to three orders of magnitude larger than for protein substrates and allow setting assays with an appropriate substrate concentration using standard phosphate detection approaches. In addition, short synthetic peptides are inexpensive and easy to obtain. Nonetheless, although phosphopeptide substrates are clearly useful in exploring interactions in the immediate vicinity of the phosphatase active site, they are unable to probe distant (allosteric) sites. 

Comparative Peptide Detection and Identification using Two-Dimensional Chromatography (SCX/Ci8) on an LC-Chip-MS System... 

Fluorescamine (Fluram). Fluorescamine was developed from the study of ninhydrin chemistry as a reagent for detecting amino groups (Udenfriend et al., 1972) and has been successfully used in the reversed phase chromatographic analysis of proteins and peptides using an on-line post-column detection system. This reagent requires two separate pumps for the delivery of the mobile phase buffer and the reagent and this method may therefore be considered to be less... 

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