Peptide nucleic acid oligos

Follow-up on vanished companies and products is difficult, unless they are acquired (Table 2). For example. Biotech Research Laboratories and their peptide nucleic acid oligos (PNAs) were acquired by Boston Biomedica, Inc. Tracking BRL and its specifically targeted PNAs to BBI is simplified by web-page links, which are easy to follow for the reader with a modest knowledge of computer use. 

PNA oligomer Peptide nucleic acid oligo (Fig. ID) manufacturers include Boston Probes (www.bostonprobes.com) the Danish company Pantheco (www.pantheco.com), and Research Genetics (www.resgen.com). These, like morpholino analogs, resist nuclease digestion. 

In 2006, Pritz and coworkers prepared the peptide nucleic acid oligo mers via stepwise PyBOP-mediated coupling of the tautomerizable hetero cycle, and Sj Ar displacement with the amine nucleophile (06TL5893). 

In addition, Dose and Seitz (2005) employed native chemical ligation to synthesize peptide nucleic acids (PNAs) by linking shorter segments of PNAs to make long contiguous strands, which could not be made through typical oligo synthesis procedures. 

An array of oligonucleotide which is composed of 20 80-mer oligos, or peptide nucleic acid probes, is synthesized either in situ (i.e., on-chip) or using conventional synthesis followed by on-chip immobilization. The resultant DNA array is then exposed to the labeled sample of DNA, hybridized, and the identity/abundance of complementary sequences determined. This method was developed at Affymetrix, Inc. and called DNA chips. Today, oligonucleotide-based chips are manufactured by many companies using alternative in situ synthesis or depositioning technologies. 

These applications require the use of FON probes resistant to nucleases. ONs with modified backbones such as oligo-2 -0-methyl-2 -deoxyribonucleotides (19), oligo-a-deoxy ribonucleotides (29), peptide nucleic acids (33) and locked nucleic acids (LNA) (50) (Fig. 4) are often used because of the commercially available building blocks. They form, with the RNA targets, hybrids that are not RNAse H substrates unless they can stimulate degradation of the targets rather than report on their presence. 

A Amino acids and peptides B Nucleic acids and polynucleotides C Oligo- and polysaccharides... 

Various types of libraries, such as the peptide hbrary, are widely used. Among them, the nucleic acid hbrary is among the most interesting, because nucleic acids exhibit seh-rephcation. Figure 6.18 shows an example where the oligo(nucleic acid) with the best affinity to thyroxine is selected from a random hbrary. In this example, a PCR that multiplies nucleic acid was used. 

The peptide was synthesized by the Peptide Synthesis Facility at Queens University. The DNA was provided by the Nucleic Acid Synthesis Facility at the University of Calgary. The peptide MRSRSPSRSKSPMR was dissolved in aqueous (90%/10% H2O/D2O) solution to a final concentration of 5 mM, the pH of the solution was adjusted to 6.5. The oligo-nucleotide d(T6C4A6) was dissolved in aqueous (90%/10% H2O/D2O) solution with lOOmM phosphate buffer (pH 6.5) and 150mM KCl to a concentration of 2.5mM. Aliquots of the peptide stock solution were added to the d(T6C4A6) solution for the titration experiments. 

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