Peptide- and protein-polymer conjugates

Synthetic Aspects of Peptide- and Protein-Polymer Conjugates in the Post-click Era 3... 

Unfortunately, utilization of copper(i) to control the regioselectivity yields several problems. Although only catalytic amounts are required, copper can induce cytotoxic effects and protein denaturation, ° which can complicate bioconjugation by CuAAC for applications in vitro and in vivo. In addition, copper salts are often rather difficult to remove from the reaction products. Nevertheless, advantages like high selectivity, mild reaction conditions, and almost quantitative product formation have proven CuAAC highly beneficial for the preparation of site-specific peptide- and protein-polymer conjugates. 

Besides the chemoenzymatic approaches described above, where functional groups are added to allow for conjugation with other macromolecules, enzymes demonstrate other possibilities to obtain peptide- and protein-polymer conjugates, which are normally difficult to obtain by common chemical synthetic strategies. Enzyme responsiveness can be used to modulate bioconjugate properties in order to create hybrid materials with... 

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