Pea seedling

A. D. Boulter, J. J. Jeremy, and M. Wilding, Amino acids liberated into the culture medium by pea seedling roots. Plant and Soil 24 121 (1966). 

Table 3 Susceptibility of Yeast Species and Bacterial Strains from Pea Seedlings Against the Heterocyclic Nonprotein Amino Acid P-I.soxylinonyl-Alanine (PlA) ...

Diamine oxidase (pig kidney, human pregnancy plasma, pea seedlings) 261 Dihydrolipoyl transacetylase (Escherichia coli)[27] Dipeptidyl carboxypeptidase[281 DNA polymerase-a (human)1291... 

In different organs of the rat [128], Ehrlich ascites tumor cells [144], trout testis [127], calf thymus [145], and carp testis [146], H4 is modified mainly as the N -dimethyllysine, while H3 is modified as N -monomethyllysine, N -dimethylly-sine and N -trimethyllysine with the N -dimethyllysine predominating. Pea seedling H4 is not methylated and H3 exits as N -mono- and N -dimethyllysine with N -trimethyllysine not being detectable [147,148]. 

RusseU DW, Conn EE (1967) The cinnamic 4-hydroxylase of pea seedlings. Arch Biochem Biophys 122 256-258... 

RusseU D (1971) The metabolism of aromatic compounds in higher plants X. Properties of the cinnamic 4-hydroxylase of pea seedlings and some aspects of its metabolic and experimental control. J Biol Chem 246(12) 3870-3878... 

The first of these enzymes has been studied the most thoroughly. Its activity has been detected in many sources, and purified preparations have been obtained from calf and beef liver,348-349 rat tissues,3498 hen oviduct,350 pea seedlings,351 Cryptococcus laurentii,352 and Aerobacter aerogenes,353 Extensive purification of the liver enzyme was achieved.349... 

More-detailed studies of enzyme specificity towards the structure of the nucleoside residue have been performed with the sucrose synthetase of pea seedlings,339,384,501,502 and with wheat-germ arbutin synthetase.339,364,503 Neither enzyme can use the cytidine,504 isocyti-dine, or N3-methyluridine derivatives as substrates. On the other hand, the a-D-glucopyranosyl pyrophosphate esters of 4-thiouri-... 

Sucrose synthetase from pea seedlings seems to be more specific than similar enzymes from other plants. Unlike them, it cannot accept thymidine 5 -(a-D-glucopyranosyl pyrophosphate) as a substrate.364... 

A polyisoprenyl phosphate has been shown to act as an acceptor for D-mannose and 2-acetamido-2-deoxy-D-glucose in mung beans347 and peas.348 Similar polyisoprenyl phosphates that accept glycosyl groups have been reported in soy bean, wheat germ, and pea seedlings,349 Pha-... 

All of the core histones share a conserved 65-residue histone fold.27 28 The arginine-rich histones have a strongly conserved amino acid sequence, histone H4 from pea seedlings differing from that of the bovine thymus by only two amino acids. On the other hand, the lysine-rich HI is almost species-specific in its sequence. Differentiated tissues contain at least seven variant forms of histone HI including proteins designated HI0, Hit, and H5 29-31... 

The conversion of D-mannose 6-phosphate into D-mannosyl phosphate is catalyzed by phosphomannomutase, an enzyme distinct from phosphoglucomutase. Both enzymes have been detected by Mathe-son705 in cassia seeds, mung beans, orchid tubers, and pea seedlings. These enzymes from cassia seeds have been separated from one another by chromatography on DEAE- and O-phosphono-cellulose columns, and further characterized. Phosphomannomutase from animal sources requires the presence of either D-galactose 1,6-bisphosphate or D-mannose 1,6-bisphosphate for activity.708 D-Mannosyl phosphate may then be enzymically transformed into GDP-D-mannose in the... 

In vitro cell free microsomes (from pea seedlings and 4-T-Cinnamic acid 3-T-4-Hydroxycinnamic acid 85-90 107... 

The ether fractions of the first and second partitions were pooled, those of the next two were pooled, and this procedure was continued until the 12 fractions were in six groups. Each of the pooled ether fractions was dried overnight with anhydrous sodium sulfate. The ether solution was decanted and evaporated to dryness. The residue from each ether extract was dissolved in 5 ml. of water and an aliquot assayed with Little Marvel pea seedlings. 

Hormone-treated pea seedlings generate two physically distinct cellulases (EC 3.2.1.4), with similar substrate specificities, Km values, and inhibitor sensitivities. They may be effectively separated by sequential extraction with buffer and salt and they appear to possess identical active sites but different apoprotein structures. The question arises of why this tissue should elaborate two hydrolases which catalyze the same reactions. The cellulase that forms first is synthesized by and accumulates in vesicles, where it would never encounter cellulose, while the other is concentrated on the inner wall microfibrils. It is suggested that only the latter cellulase functions to hydrolyze cellulose. A precursor/ product relationship between them could explain their distribution and developmental kinetics, but physical and chemical differences mitigate against this interpretation. 

Figure 3. Development of BS and BI cellulase activity in apices of pea seedlings. Intact seedlings were sprayed with the auxin analogue 2,4-D and decapitated seedlings were painted with the natural auxin IAA with or without an inhibitor of DNA synthesis, FUdR. All treatments resulted in massive swelling at the pea apex because of cell expansion cell divisions also occurred, but not in the presence of FUdR (6). Cellulases were extracted as described in Figure 1 and assayed in unpurified form.

Turowski P. N. McGuirl M. A. Dooley D. M. Intramolecular electron transfer rate between active-site copper and topa quinone in pea seedling amine oxidase. J. Biol. Chem. 1993, 268, 17680-17682. 

Brian, P.W., Hemming, H.G. "The effect of gibberellic acid on shoot growth of pea seedlings." Physiol. Plantarum, 1955, 86, 669-681. 

ABA is metabolized via the unstable intermediate 6 -hydroxymethyl -ABA, more recently called hydroxyabscisic acid (HOABA) by Hirai et al. (45), to phaseic acid (PA). In certain plants the latter compound is further converted to 4 -dihydro-phaseic acid (DPA) which accumulates as the end product. The ABA-> PA DPA pathway (Figure 4) operates in beans (46), in pea seedlings (47), in ash seeds (48), in castor bean (49), and... 

Figure 4. Effect of inhibitors of RNA and protein synthesis on ethylene production in subhook sections of etiolated pea seedlings.

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