Primer Express software

The PCR primers and the fluorogenic probes for the studied targets were designed using the Primer Express Software 1.7 (PE Applied Biosystem, Foster City, CA, USA). To normalize the quantitative data, specific probes for the TATA-bind-ing protein mRNA were used as an internal control. 

We used Primer Express software (ABl) in calculating the T of primers and probes. Any program should work, just make sure to use the same program and conditions for calculating T , values of both the primers and probes. 

Primer Express software v2.0 was used to select TaqMan primer and probe sequences for the Y. pestis pla gene. 

Brenner, M., Dunlay, T., Davidson, M. (n.d.). Fluorescence in situ hybridization Hardware and software implications in the research laboratory. Retrieved October 7, 2008, from Molecular Expressions Microscopy Primer Web site http //www.microscopyu.com/articles/ fluoresce nce/insitu/brenne rinsitu.html... 

Figure 9 RT-PCR analysis of cytokine mRNA expression in the spleens of DBA/2 mice immunized with casein/CKA. Total RNA was extracted from pooled spleens of S DHA/2 mice or CVE-administercd mice 3 weeks after immunized using RNAzol and reverse-transcribed, and the cDNA concentration was adjusted by co-amplification of two fold serially diluted cDNA and constant amounts of control plasmid pMCQ with [5-actin specific primers. Cytokine expression patterns of IL-12, IFNy, IL-6, and 11.-10 were determined by PCR using murine cytokine-specific primers. The PCR products were separated in 2 % agarose gel and visualized by ethidium bromide staining. Relative quantification of the amounts of the RT-PCR products was performed using a Computing Densitometer and NIH Image software,...

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