Particle-tracking autoradiography

At-MNDP 31-33, 36, 108), whose mechanism of intracellular localization is related to the presence of oncogenically expressed tumor-membrane alkaline phosphatase isoenzymes 42, 108), has been demonstrated strikingly effective in an animal tumor model 33, 34, 38, 39). It has also served as a concomitant analytical probe for identifying the intracellular locus of radiotherapeutic action of this class of drug by a-particle track autoradiography 33,106-109). Phase I and II human therapeutic trials are shortly envisaged 33, 34). 

Key words Mo-B alloy, Grain boundary segregation, Precipitates, Particle-tracking autoradiography (PTA), Tritium autoradiography (TA). 

Some relevant terms for activation analysis are activation analysis, neutron activation analysis (NAA), instrumental neutron activation analysis (INAA), neutron activation analysis with radiochemical separation (RNAA), photon activation, neutron capture prompt gamma activation analysis (PGAA), charged particle activation, autoradiography, liquid scintillation counting, nuclear microprobe analysis, radiocarbon (and other element) dating, radioimmunoassay, nuclear track technique, other nuclear and radiochemical methods. Briefly, the salient features of some of the more popular techniques are as follows ... 

In other studies, Edwards (248), Fairchild, Tonna, and Seibold (257, 258) and Frigero (293) have employed activation analysis low-background radiographic techniques to measure the boron distribution within cells in order to use Boron-10 in clinical trials of an experimental therapuetic procedure, that is, neutron capture therapy. These particular cellular distribution studies were carried out on mouse spleen, and, after the activation, the materials were exposed to photographic nuclear track emulsions of Eastman Kodak Type NTA. The countable a-particle tracks obtained in these experiments showed that concentrations of B-10 of 60 Mg/gm, the amount that can be safely delivered to tumors in human subjects, could be readily determined. In other applications of activation analysis and autoradiography, Vincent (963) and Vincent, Haumont and Roels (964) made microscopic determinations of sodium in compact bone and of bone phosphorus. 

Plutonium particles were located and identified within alveolar macrophages by autoradiography. The paths of the a particles emitted from the plutonium within the cell formed a star of tracks of reduced silver halide grains (Morrow and Casarett 1961, Sanders 1970). 

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