Packed Capillary Column and Unified Chromatography

Small-diameter packed columns offer (17) the substantial advantages of small volumetric flow rates (1-20 (p.L min )), which have environmental advantages, as well as permitting the use of exotic or expensive mobile phases. Peak volumes are reduced (see Table 1.1), driven by the necessity of analysing the very small (pico-mole) amounts of substance available, for example, in small volumes of body fluids, or in the products of single-bead combinatorial chemistry. 

The increasing use of microcolumns has moved chromatography towards uniflca-tion. Giddings was the first to point out (18) that there was no distinction between 

Column internal diameter Volumetric flow rate Injection volume UV-deteaor cell volume Sensitivity improvement 

GC Peti oleum gases Gasoline Packed capillary (ODS) FID 

SFC Petroleum wax Atmospheric and vacuum residues Lube oil additives Open tubular FID 

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