P-aminophenyl acetate

Figure 11.4 The effect of CTAB on the hydrolysis of four esters expressed as a ratio ( / o) of the first order rate constants obtained in the presence (/c ) and absence (/cq) of the surfactant. , EPAB A, PAPA , PNPA B, EPNB all in Delory and King s buffer O, EPAB in Sorensen s glycine buffer. The esters used were ethyl p-aminobenzoate (EPAB), p-nitrophenyl acetate (PNPA), ethyl p-nitrobenzoate (EPNB) and p-aminophenyl acetate (PAPA). From Meakin et al. [28].

A mixture of methyl 2-(p-aminophenyl)propionate (44.75 g), methallyl chloride (34 g) and pyridine (30 ml) in isopropanol (400 ml) is boiled during 30 hours. The solvent is removed in vacuo and the residue is taken up into water and ether. After separation, the organic phase is washed repeatedly with water, after which it is dried and concentrated in vacuo. The resulting oil is fractionally distilled in vacuo (0.1 mm Hg 5 g of oil essentially consisting of methyl 2-(p-aminophenyl)propionate are collected at 115°-120°C 30 g of oil consisting of a mixture of mono- (80%) and disubstituted (20%) amines is collected at 128°-130°C. This oil is used to prepare the hydrochloride, which is recrystallized from ethyl acetate, to give white crystals (22.7 g) melting at... 

An affinity adsorbent was prepared from the p-aminophenyl a-isomalto-side and CNBr-activated Sepharose 4B by reacting 0.03 g of the isomaltoside with 4 g of activated Sepharose 4B, following the procedure described in an earlier section. The final product was washed with 100 mL of 0.1 M acetate buffer of pH 4, followed by 100 mL of 0.1 M hydrogen-carbonate buffer of pH 8, and then transferred to a column (1.5 cm x 20 cm) and washed further with 0.02 M phosphate buffer of pH 7 in saline. The washed column was stored in a cold room until used to prepare the reactive protein. 

AMINOPHENYL)(4-IMINO-2,5-CYCLOHEXADIEN-l-YLIDENE)METHYL), MONOCHLORIDE see RMK020 p-AMINOPHENYLMERCURIC ACETATE see ABQOOO... 

The intermediate in the Gabriel-Posner synthesis of quinazolin-2(lH)-one (fusion of o-aminobenzaldehyde with excess of urea), was found to be o-ureidobenzylidene urea (19), which then cyclized on heating, or in the presence of acid, with elimination of urea. The intermediate reacted with aniline, iV-methylaniline, and N,V-dimethylaniline to form 4-p-aminophenyl, 4-p-methylaminophenyl, and 4-p-dimethylaminophenyl quinazolin-2(lH)-ones in an unusual manner. Several quinazolin-2(li/)-ones were prepared by converting o-acylanilines into o-ureidophenyl ketones followed by cycli-zation in acetic acid medium for long periods at 55°C. A novel modification of this cyclization involved a Curtius or Hofmann reaction on 2 -benzoyl-oxanilic acid chlorides (20 = Cl) or amides (20 = NH2), respectively. 

Nitro substituents in dihydrodiazepinium salts can be reduced by means of iron and acetic acid without reduction of the dihydrodiazepinium ring for example, p-aminophenyl-substituted dihydrodiazepinium salts have been obtained in this way.28 Although 6-nitro groups can also be reduced by metal and acid, it is not a satisfactory method for the preparation of 6-aminodihydrodiazepinium salts since the products apparently form complexes with the metal. 6-Amino derivatives are most conveniently prepared by catalytic reduction of the related nitro compound using either... 

Hi. BrAcEK [N- N"-Bromoacetyl-N -DNP-h-lysyl)-p-aminophenyl-/3-lactoside]. To a solution of bromoacetic acid (1 mmole) in p-dioxane (3 ml) are added A-hydroxysuccinimide (1.1 mmoles) and dicyclohexylcarbodiimide (1.5 mmoles). After reaction at room temperature for 90 min, the precipitated di cyclohexyl urea is removed by filtration and washed with dimethylformamide the combined filtrates are added to a solution of EK compound" (0.25 mmole) in dimethylformamide (2 ml). After 2 hr at room temperature, the solution is flash-evaporated to dryness, dissolved in the upper phase of n-butanol water glacial acetic acid (4 5 1, 10 ml), and separated for 50 transfers by countercurrent distribution. The upper phase from tubes 31 through 45 and lower phase from 34 through 42 are combined and flash-evaporated to dryness. The residue is triturated with ether and dried. The tritiated form of BrAcEK is obtained by using... 

Hydroxy-9-phenyl-fluorene (0.02) HCl (0.6) Acetic acid 115 1 9-p-Aminophenyl-9-phenylfluorene (80%) 325... 

An intimate mixture consisting of 1 molecular equivalent of the zinc salt of o-aminophenyl selenide and 2 equivalents of p-nitrobenzoyl chloride is fused for an hour at 100° C. The cooled melt is then pulverised and washed successively with water and sodium carbonate solution. Repeated crystallisation from glacial acetic acid gives pale yellow lustrous needles, M.pt. 211c C. (corr.). 

Yoda,104 after reacting non-reducing sugars and polyhydric alcohols with periodate, used a mixture of manganous sulfate and bis-(p-dimethyl-aminophenyl)methane in acetic acid to detect the spots sensitivity, 5 to 50 micrograms. 

The solution of N-acetyl-N-(3-aminophenyl)-p-amino-isobutyric acid was filtered after standing overnight, mixed with an equal volume of acetic acid, and there after with a solution of 275.0 g iodine monochloride and 200.0 g sodium chloride in 1 L water. The mixture was kept at 50°C with stirring for 48 h. Light brown crystals of crude N-acetyl-N-(2,4,6-triiodo-3-aminophenyl)-p-amino-isobutyric acid precipitated, were filtered off, washed with water, and dried. They weighed 195.0 g (84.5% yield based on N-acetyl-N 3-nitrophenyl-p-amino-isobutyric acid). 

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