Oscillating centrifuges

Pi re 8.33 Schematic illustration of an oscillating centrifuge [Courtesy of Kiauss Mafiei Conqiany, Germai ]... 

Link-Suspended Basket Centrifuges In centrifuges with diameters larger than 762 mm (30 in), the basket, curb, curb cover, and drive form a rigid assembly flexibly suspended from three fixed posts (also known as a three-column centrifuge). The three suspension members may be either chain hnks or stiff rods in ball-and-socket joints and are spring-loaded. The suspended assembly has restrained freedom to oscillate to compensate for a normal out-of-balance condition. The drive is vertical with more efficient power transmission compared to the base-bearing type. 

Vibrating screens, inclined and horizontal oscillating screens centrifugal screens. 

M.A. Van Nierop, M.H. Moys, Premature centrifuging, oscillation and axial mixing in industrial grinding mill load, Minerals Eng. 11 (1998) 437-445. 

Two thousand parts by volume of an aqueous culture medium (pH 7.2) comprising 0.5% of glycerol, 0.5% of polypeptone, 0.5% of yeast extract and 0.3% of meat extract is inoculated with Escherichia coli Rll (IFO-13560). The medium is incubated at 37°C under aeration for 18 h. The culture broth is subjected to centrifuge to recover 4.4 parts of wet cells. The cells are suspended into 17.6 parts by volume of 0.05 M phosphate buffer (pH 7.0). The suspension is subjected to ultrasonic oscillation (Kaijo Denki Co., Ltd. T-A-4201, 4280-type, 2A) to disintegrate the cells, followed by removing the debris (insoluble materials) by centrifugation, whereby 17 parts by volume of crude enzyme solution is obtained. 

The two cases which arise in diatomic molecules are rotational predissociation and electronic predissociation the latter case applies only to excited electronic states. We deal first with rotational predissociation, with can arise for either ground or excited states. The potential energy curve shown for a Morse oscillator in section 6.8 is for a rotationless (./ = 0) molecule. For a rotating molecule, however, we must add a centrifugal term to the potential,... 

In contrast to bulk foam, in a porous medium the majority of films move separately so their rupture under certain critical pressure does not occur simultaneously, i.e. the films are not affected by the rupture of other films (there is no collective effect). On the other hand, moving films are subjected to the oscillation of thickness as well as to other mechanical effects. Hence, their critical pressure should be lower than that of the static foam and should depend also on the rate of movement due to the dynamic effects. Analogous reduction in the critical pressure is observed when a bulk foam advances [47] and when a foam is placed in a centrifugal field [183]. The influence of the dynamic effects on the critical pressure has been explained by the model of Jimenez and Radke [175]. 

The source of enzyme activity was mycelium from Streptomyces clavuling-erus. The mycelium were disrupted by sonic oscillation. The supernate was clarified by centrifugation and for best results desalted and equilibrated on a Sephadex G-10 column with 0.1 Af Mops buffer (pH 7.0) in 25% (v/v) aqueous glycerol containing 20 mAf MgCl2, 30 mAf 2-mercaptoethanol, 2 mAf dithio-threitol, and 5 mAf EDTA. 

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