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Organisms of Interest

Since the target for the use of these products is typically hospital or food preparation areas, the main organisms of concern are pathogenic bacteria such as methycillin resistant Staphylococcus aureus (MRSA), Escherichia coli and other enteric organisms. Other organisms may include dust mites and anti-viral applications such as severe acute respiratory syndrome (SARS). [Pg.8]

SARS is also a disease in the news over recent years and a plastic resistant to SARS has been mentioned in some journals. From the Centre for Disease Control www.cdc.gov) fact sheet on SARS dated 13 January 2004, SARS is a viral respiratory illness caused by a coronavirus, called SARS-associated coronavirus. [Pg.9]

The main way that SARS seems to spread is by close person-to-person contact. The virus that causes SARS is thought to be transmitted most readily by respiratory droplets (droplet spread) produced when an infected person coughs or sneezes. Droplet spread can happen when droplets from the cough or sneeze of an infected person are propelled a short distance (generally up to three feet) through the air and deposited on the mucous membranes of the mouth, nose, or eyes of persons who are nearby. The virus also can spread when a [Pg.9]

A plastic article that may have a bioeide or is itself resistant to SARS will therefore be likely to have little or no effect on the spread of the disease. [Pg.9]


The biodistribution of the ideal radiopharmaceutical would show extremely rapid distribution via the circulatory system to the organs of interest and htfle distribution to others. It would be rapidly extracted by the organ or tissue of interest differentially in a way that reflects the disease process of interest. Ideally, abnormahties should be defined by substantial increases in the concentration of the agent because lesions defined by a decrease from surrounding concentrations, ie, cold-spot imaging, are more difficult to image. [Pg.473]

Figure 2.1. Schematic illustration oftwo-dimensional gel electrophoresis. Proteins are extracted from the organism of interest and solubilized. The first dimension separates proteins based on isoelectric point. The pi strip is reduced and alkylated and applied to an SDS-PAGE gel for separation by molecular weight. Proteins canbe visualized using a number of staining techniques. Figure 2.1. Schematic illustration oftwo-dimensional gel electrophoresis. Proteins are extracted from the organism of interest and solubilized. The first dimension separates proteins based on isoelectric point. The pi strip is reduced and alkylated and applied to an SDS-PAGE gel for separation by molecular weight. Proteins canbe visualized using a number of staining techniques.
Figure 7.1. Protein expression mapping using an antibody array. The antibody array consists of monoclonal antibodies specific for a set of proteins in the organism of interest gridded onto a filter. To determine if a protein is expressed under the conditions being tested, a crude lysate is obtained and the proteins within the lysate are labeled with a fluorescent tag. The lysate is applied to the filter and the proteins are allowed to bind to the relevant antibody. Bound proteins are visualized via the fluorescent tag. Figure 7.1. Protein expression mapping using an antibody array. The antibody array consists of monoclonal antibodies specific for a set of proteins in the organism of interest gridded onto a filter. To determine if a protein is expressed under the conditions being tested, a crude lysate is obtained and the proteins within the lysate are labeled with a fluorescent tag. The lysate is applied to the filter and the proteins are allowed to bind to the relevant antibody. Bound proteins are visualized via the fluorescent tag.
When the bacteria to be detected are less than 1% of the total population in a sample, IFAs cannot be used because of interference from unrelated particles that are concentrated when large volumes of sample are filtered. To overcome this problem, the organism of interest may be concentrated by immunomagnetic separation.10,51 62 For this procedure magnetic beads coated with monoclonal or polyclonal antibodies are mixed with the sample. The beads are collected with a magnet, and the cells attached to the beads then are removed, enumerated, and identified by IFAs. [Pg.7]

The interplay of the processes of absorption, distribution, metabolism and excretion result in changes in concentration of the test chemical in different organs with time. With regard to the practical concerns of monitoring human exposure, the organ of interest is the blood. Blood can be considered a central compartment. Determining the concentration of the chemical in plasma gives one an assessment of exposure. Mathematical formulas are used to quantitatively describe this exposure. [Pg.714]

Transfer the organ of interest into a sterile petridish. [Pg.54]

Destination vector. A vector for expression of a cloned ORF in an organism of interest. This vector should have the ccdP> cassette at the position where the ORF is to be inserted, as is the case for a Donor vector see Note 3). [Pg.17]

Genomic clone A host cell (usually bacterium) with a vector containing a fragment of genomic DNA from a different organism of interest... [Pg.37]

In the following pages, we describe some experiments run in our High Pressure laboratory, in order to show how CO2 treatment technology can be applied to micro-organisms of interest for the agro-alimentary industry. [Pg.635]

From a freshly killed animal, dissect out the organs of interest and gently wipe excess blood from the outside... [Pg.268]

Suppose that you are seeking bacterial mutants with altered triose phosphate isomerase (TPI). The organism of interest is known to use the glycolytic pathway with the production of lactate. [Pg.278]


See other pages where Organisms of Interest is mentioned: [Pg.233]    [Pg.475]    [Pg.483]    [Pg.283]    [Pg.416]    [Pg.10]    [Pg.81]    [Pg.82]    [Pg.32]    [Pg.32]    [Pg.154]    [Pg.268]    [Pg.960]    [Pg.246]    [Pg.621]    [Pg.124]    [Pg.595]    [Pg.552]    [Pg.287]    [Pg.326]    [Pg.207]    [Pg.9]    [Pg.233]    [Pg.165]    [Pg.178]    [Pg.322]    [Pg.48]    [Pg.61]    [Pg.65]    [Pg.10]    [Pg.202]    [Pg.537]    [Pg.51]    [Pg.204]    [Pg.21]    [Pg.23]    [Pg.910]    [Pg.329]    [Pg.5]    [Pg.475]   


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