Opus analyzer

The Opus system consists of the Opus analyzer (Fig. 26), assay-specific test modules (Fig. 27) and calibrator sets. This system performs in vitro quantitative and qualitative fluorescent immunoassays developed by PB Diagnostic Systems, a joint venture of the Polaroid Corporation and Behringwerke AG. 

To operate the Opus analyzer, the operator selects a test and provides other information through an interactive touch screen/LCD display. When prompted, the operator inserts one assay-specific test module per test into the loading port of the instrument. The loader transports the test module to a 20 position incubated rotor where the test module is equilibrated to 37 C. The analyzer prompts for sample cup and pipette tip trays. Once these trays are supplied, the analyzer automatically picks up a new pipette tip, aspirates the sample and dispenses the sample onto the test module. For certain assays the pipettor also transports conjugate, substrate or other reagents from the test module wells to the test module dispense port. After processing, the test module is rotated to the read position where the fluorimeter takes measurements. The rotor then moves the used test module to the loader/ ejector where it is... 

High-throughput measurements are performed in a Tensor 27 spectrometer connected to a HTS-XT system (Bruker Optik). Each supernatant mixture (3 pL) is transferred to a 384-well microtiter plate equipped with a silicon plate for IR transmittance. Every sample is measured with a resolution of 8 cm-1 and a scan number of 10, so the total time for the analysis of each sample is 8.9 s, allowing a throughput of >9000 samples per day. The resulting spectra are analyzed with Opus and Opus Lab software. The first 14 samples are used for calibration, and the remaining... 

In later papers they extended the theory to cover multiple scattering effects [241,242]. They also investigated neural network recognition of particle size distribution by ultrasonic spectroscopy [243] for measuring high concentration suspensions. This work formed the basis for an on-line ultrasonic size analyzer (OPUS) which is available from Sympatec (Figure 10.15). 

The continuous access feature allows the operator to have access to the analyzer at virtually all times. Even while assays are in progress, the operator can access reports and enter up to ninety-nine test requests. A queue of tests may consist of any combination of the Opus assays. 

The reagents used for the Opus system are totally self-contained in the test modules. There are two types of assay methodologies used with the analyzer multilayer film assays and fluorogenic ELISA assays. 

Analyzer Ektachem 700 Ektachem DT-60 Reflotron Cobas Ready, Spotchem Seralyzer Stratus Opus... 

A near-infrared spectrometer (Equinox 55 from Bruker) was used with OPUS software. The near-infrared spectra were recorded in the 4000-8000 cm range. For transmission analysis, polymer coatings (from 50 pm to 2 mm thick) were analyzed after debonding of the coating from the substrate by bending the coated substrate, and background spectra in air were recorded. For each analysis, 128 scans were collected at 4 cm resolution. 

This chapter deals with the discussion of all OPUS data processing and manipulation procedures, which are shown in Fig. 10.1. These functions allow you to transform or analyze the spectral data after it has been acquired. The results of all operations are temporary files, i.e. existing only in the computers memory, called work files that must be saved explicitly. If you exit OPUS without saving the work files to disk, the results of the data manipulation will be lost. OPUS warns you in case you attempt to shut down the program. 

Foiirier Transform Spectroscopy Infrared (ETlK) Infrared spectra were obtained using a Bruker Optik GmbH Tensor 27 FT-IR, controlled by an Opus/IR software, which uses Fourier transform to process data. Samples were analyzed as liquid films in KBr cells and dry KBr pellets. 

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