Multilayer film assays

The reagents used for the Opus system are totally self-contained in the test modules. There are two types of assay methodologies used with the analyzer multilayer film assays and fluorogenic ELISA assays. 

The multilayer film assay is based on the principle of a competitive immunoassay. These assays are used to determine concentrations of low molecular... 

Calibration cycle Every 3 months Every 3 months Not required only by manufacturer Every 3 months Every 7-30 days Every 30-60 days Multilayer film assays every 6-8 weeks, EMIT assays every 2 weeks... 

E207 losefsohn, M. and Hicks, J.M. (1985). Ektachem multilayer dry-film assay for ammonia evaluated. Clin. Chem. 31, 2012-2014. 

The OPUS system has dry reagents impregnated in a coated multilayer film chip encased within a plastic bar coded test module. No external or supplementary reagents are required. A serum or plasma specimen is applied to the test module by the instrument and the fluorescence intensity from the module is measured after an assay-specific time interval. 

El 17 Kubasik, N.P., Sine, H.E. and D Souza, J.P. (1983). Evaluation of several analytes assayed using multilayered dry film reagent slides with the Eastman Kodak Ektachem 400 analyzer. Clin. Chem. 29, 1246, Abstr. 514. 

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