High-mannose glycans

A fourth family of A-glycans are referred to as hybrid glycans. These glycans share structural features of the high mannose and complex-type families. They usually retain two mannoses on the 6-arm of the trimannosyl core whilst complex-type antennae are elaborated on the 3-arm. Hybrid structures are frequentiy bisected and may also be core fucosylated (Fig. 15.1). It should be noted that whilst core fucosylation is common in complex-type, hybrid and truncated glycans, it is rarely found in high mannose glycans. 

Bolmstedt, A.J., et al. 2001. Cyanovirin-N defines a new class of antiviral agent targeting N-linked, high-mannose glycans in an oligosaccharide-specific manner. Mol Pharmacol 59 949. 

Figure 20.3 ESI-MS-MS spectrum of a 2-AMAC-derivatized high-mannose glycan with Man 8. Reprinted from [55] with permission. 1999, John Wiley and Sons Ltd.

Astoul, C. H., Peumans, W.J., Van Damme, E. J.M., Rouge, P. Accessibility of the high-mannose glycans of glycoprotein gpl20 from human immunodeficiency vims type 1 probed by in vitro interaction with mannosebinding lectins. Biochem. Biophys. Res. Com-mun. 2000, 274, 455 160... 

Figure 11.9. (a) PSD mass spectrum of the MALDI-produced [M + Na]+ ions of a high-mannose glycan (Man)6(GlcNAc)2 that was released from ovalbnmin, and (b) the fragmentation scheme. (Reproduced from ref. 28 by permission of Elsevier, copyright 2000 American Society for Mass Spectrometry.)... 

Type 1 FimH High-mannose glycans 2VCO 162, 163... 

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