Of reducing oligosaccharides

Guttman, A., Chen, F.A., Evangelista, R.A. and Cooke, N. (1996) High-resolution capillary gel electrophoresis of reducing oligosaccharides labeled with l-aminopyrene-3,6,8-trisulfonate. 

The oxidation behavior of reducing oligosaccharides is essentially a combination of the patterns exhibited by monosubstituted derivatives of monosaccharides and by glycosides. In general, these merged patterns are sufficiently characteristic under suitable conditions to permit unambiguous characterization... 

S. Honda, K. Kakehi, and Y. Kubono, Prevention of overoxidation in periodate oxidation of reducing oligosaccharides by their conversion into 1,5-anhydroalditol derivatives, Carbohydr. Res., 75 (1979) 61-70. 

For labeling larger amounts of reducing oligosaccharides (from more than 20 tg of glycoprotein), UV chromophore (Kakehi et al. 1991) can also be used. The mild reaction conditions do not cleave the sialic acids off the oligosaccharides, and the excess of UV chromophor is removed after the reaction by simple extraction. UV chromophor is not available in retail and its production takes a few days (Kakehi et al. 1991). 

The properties and reactivity of reducing oligosaccharides are comparable to the properties and reactivity of monosaccharides. 

Figure 6. The cumulative release of reducing oligosaccharides from dex-MA hydrogels (DS 4, initial water content 70% w/w) containing dextranase (0.03 U/g gel ( ), 0.1 U/g gel (B), 1 U/g gel (A).

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