Glycosaminoglycan oligosaccharides

Saitoh H, Takagaki K, Majima M, Nakamura T, Matsuki A, Kasai M, Narita H, Endo M. Enzymic reconstruction of glycosaminoglycan oligosaccharide chains using the transglycosyla-tion reaction of bovine testicular hyaluronidase. J. Biol. Chem. 1995 270 3741-3747. 

Zamfir, A., Seidler, D. G., Schonherr, E., Kresse, H. and Peter-Katalinic, J. On-line sheathless capillary electrophoresis/nanoelectrospray ionization-tandem mass spectrometry for the analysis of glycosaminoglycan oligosaccharides. Electrophoresis, 25, 2010, 2004. 

Polysaccharide lyases can be combined with separation methods such as chromatography and electrophoresis for the preparation of glycosaminoglycan oligosaccharides for biological evaluations as well as for disaccharide analysis, oligosaccharide mapping and polysaccharide sequencing. 

Karst NA, Linhardt RJ (2003) Recent chemical and enzymatic approaches to the synthesis of glycosaminoglycan oligosaccharides. CurrMed Chem 10 1993-2031 Yeung BKS, Chong PYC, Petillo PA (2002) Synthesis of glycosaminoglycans. J Carbohydr Chem 21 799-865... 

Wolff, J. J. Laremore, T. N. Busch, A. M. Linhardt, R. j. Amster, I. J. Influence of charge state and sodium cationization on the electron detachment dissociation and infrared multiphoton dissociation of glycosaminoglycan oligosaccharides. J.Am. Soc. Mass Spectrom. 2008, 19, 790-798. 

Differences in the structure of glycoproteins can manifest themselves in a number of ways. Perhaps the first point to be emphasized is that the nature of the polypeptide chain to which oligosaccharide moieties become attached is not always the same. This is a situation that we have already discussed in connection with certain of the glycosaminoglycans (see p. 440). 

Mao W, Thanawiroon C, Linhardt RJ (2002) Capillary electrophoresis for the analysis of glycosaminoglycans and glycosaminoglycan-derived oligosaccharides. Biomed Chromatogr 16 77-94... 

Glycoproteins contain covalently linked oligosaccharides that are smaller but more structurally complex, and therefore more information-rich, than glycosaminoglycans. 

N-Acetylneuraminic acid N-Acetylneuraminic acid (NANA) is a member of the family of sialic acids, each of which is acylated at a different site. These compounds are usually found as terminal carbohydrate residues of oligosaccharide side chains of glycoproteins, glycolipids, or, less frequently, of glycosaminoglycans. The]... 

---