Oligosaccharide complex

M Stefansson, M Novotny. Electrophoretic resolution of monosaccharide enantiomers in borate-oligosaccharide complexation media. J Am Chem Soc 115 11573-11580, 1993. 

I Capila, MJ Hernaiz, YD Mo, TR Mealy, B Campos, RJ Linhardt, BA Seaton. Annexin V-heparin oligosaccharide complex suggests heparan sulfate-mediated assembly on cell surface. Structure 9 57-64, 2001. 

DMF has been used as cosolvent in some glycosylation reactions, see P. V. Nikrad, H. Beier-beck, and R. U. Lemieux, Molecular recognition X. A novel procedure for the detection of the intermolecular hydrogen bonds present in a protein-oligosaccharide complex, Can. J. Chem. 70 241 (1992), and references cited therein. 

The biochemical work described in Chapter 5 indicated that the microbubble surfactant mixture actually represents a glycopeptide-lipid-oligosaccharide complex, which is reversibly held together by both hydrogen bonding and nonpolar interactions. The experiments described below were undertaken to examine in detail the surface properties of the microbubble surfactant complex at the air/water interface (ref. 361). 

Gawlitzek M, Valley U, Wagner R (1998), Ammonium ion and glucosamine dependent increases of oligosaccharide complexity in recombinant glycoproteins secreted from cultivated BHK-21 cells, Biotechnol. Bioeng. 57 518-528. 

French AD, GP Johnson, AM Kelterer, MK Dowd, CJ Cramer (2001) QM/MM distortion energies in di- and oligosaccharides complexed with proteins. Int. J. Quantum Chem. 84 (4) 416-425... 

The carbohydrate-binding specificity of purified Dolichos biflorus lectin has been studied in detail by Etzler and Kabat.108 In precipitin studies conducted on the Dolichos lectin, 87-99% was precipitated by ovarian cyst A substance, whereas only 60-70% was precipitated by hog A substance the latter result was attributed to the formation of soluble, lectin-oligosaccharide complexes. At substances were more reactive than A2 substances however, no reaction was observed with B or O substances, even following mild hydrolysis with acid or two stepwise, Smith degradations. N-Deacetylation of A substance with... 

In addition, lipid-oligosaccharide complexes called lipoteichoic acids protrude from the membrane through the peptidoglycan wall. (Figure 9.26). 

Fig. 3. Titration data obtained by DOSY experiments for (GlcNAc) when added in portions to a hevein domain-containing solution. The plot is of the change in log D (Alog D) as a function of stoichiometrically added (GlcNAc) to a hevein domain containing solution. The predicted AlogZ) values for free hevein (1 0), as well as 1 1 and 2 1 protein-oligosaccharide complexes, are indicated in the right part of the panel.

IR-MALDl enables comparably soft desorption processes and is, therefore, more appropriate for noncovalent complex analysis than UV-MALDl. This is reflected by the successful detection of intact double-stranded DNA as well as enzyme-oligosaccharide complexes in combination with glycerol as matrix which were not detectable or underwent significant fragmentation when UV-MALDI was employed [126, 127]. 

Stehle T, Harrison SC (1997) High-resolution structure of a polyomavirus VP 1-oligosaccharide complex implications for assembly and receptor binding. EMBO J 16 5139-5148... 

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