Oligosaccharide-protein complexes

Duan, X.Q., Hall, J.A., Nikaido, H., Quiocho, F.A. (2001). Crystal structures of the malto-dextrin/maltose-binding protein complexed with reduced oligosaccharides flexibility of tertiary structure and ligand binding. Journal of Molecular Biology, 306, 1115-1126. 

W.l. Weis, K. Drickamer, and W.A. Hendrickson. 1992. Structure of a C-type mannose-binding protein complexed with an oligosaccharide Aatwre 360 127-134. (PubMed)... 

In a study of the tolerability and immunogenicity of Hib vaccines, 30 volunteers aged 69-84 years were immunized with either Pedvax-Hib (a conjugate of Hib polysaccharide and an outer membrane protein complex of Neisseria meningitidis-YR -OMP) or Hib TITER (a conjugate of Hib oligosaccharide and a non-toxic mutant diphtheria toxin, CRM 197-HbOC) (4). The volunteers... 

Ng, K. K.-S., Weis, W I, Stmcture of a selectin-like mutant of mannose-binding protein complexed with sialylated and sulfated Lewis oligosaccharides. Biochemistry, 36, 979-988, 1997. 

They supply valuable information on the influence of saccharide size and structure on immuno-genicity. The use of s)uithetic oligosaccharides in the development of glycoconjugate vaccines for human use is, however, a very complex task far beyond a laboratory synthesis of a given oligosaccharide and oligosaccharide-protein conjugate. 

A general essay on column chromatography of carbohydrates was written by Capek and Stanek [123], the same authors [224] reviewed polysaccharides, and Juficova and Deyl [225] summarized chromatographic separation of polysaccharide-protein complexes. Reviews specialized on ion exchange chromatography of oligosaccharides... 

Quinternary structure (5° structure) refers to the association of one class of biomacromolecule with another class of biomacromolecule to form complexes of cellular components such as histone (DNA-protein), ribosome (RNA-protein) and glycoprotein (oligosaccharide-protein). 

In the following, the different approaches to the study of intermolecular interactions by NMR will be presented in more detail. Since most of the systems studied so far are protein complexes (with organic ligands, nucleic acids, other proteins etc.), we will limit this discussion to protein-ligand interactions. However, all the approaches discussed in the following can also be employed for studies of ligand interactions with DNA, RNA or oligosaccharides, as numerous applications show. 

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