Oligonucleotides, stability

Richards Cl, Choi S, Hsiang J-C, Antoku Y, Vosch T, Bongiomo A, Tzeng Y-L, Dickson RM (2008) Oligonucleotide-stabilized Ag nanocluster fluorophores. J Am Chem Soc 130 5038-5039... 

Guo W, Yuan J, Wang E (2009) Oligonucleotide-stabilized Ag nanoclusters as novel fluorescence probes for the highly selective and sensitive detection of the Hg2+ ion. Chem Commun 23 3395-3397... 

Hoke GD, Draper K, Freier SM, et al. Effect of phosphorothioate capping on antisense oligonucleotide stability, hybridization and antiviral efficacy versus herpes simplex virus infection, Nucleic Acid Res 1991 20 5743-5748. 

Kurreck, J., E. Wyszko, C. Gillen and V. A. Erdmann (2002). Design of antisense oligonucleotides stabilized by locked nucleic acids. Nucleic Acids Res 30(9) 1911-8. 

Synthesis and enzymatic stability of oligonucleotides consisting of isonucleosides 99PAC1143. 

The authors found that the yield of 30-mer (a product with 5—6 linkages) was not much smaller than that of 10-mer or 12-mer. These facts indicate that the stability of the complex between the oligonucleotides and the complementary template is the most important factor in determining the extent of the condensation. The strong influences of template polymer (Poly C) are demonstrated in Fig. 9, in which the elution profile is shown of the polymerization products of (2 MeIp)6 in the presence of Poly C (B) and in their absence (A). 

Recently, Switzer and co-workers have further extended the multi-stranded motifs for nucleic acids with the formation of a quintet assembly with oligonucleotides containing 2,deoxy-iso-guanosine (74). To support the quintet, metal ions larger than those appropriate for quartet stabilization were required, and Cs+ ions were found to best meet this requirement. From modeling studies, a structure in which a central Cs+ interacts with ten 02 iG atoms at a distance of 3.5 A was proposed. 

Remaut, K., Lucas, B., Braeckmans, K., Sanders, N. N., De Smedt, S. C. and Demeester, J. (2005). FRET-FCS as a tool to evaluate the stability of oligonucleotide drugs after intracellular delivery. J. Control Release 103, 259-71. 

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