Oligonucleotide hybridization

Robelek R, Stefani FD, Knoll W (2006) Oligonucleotide hybridization monitored by surface plasmon enhanced fluorescence spectroscopy with bio-conjugated core/shell quantum dots. Influence of luminescence blinking. Phys Status Solidi A-Appl Mater Sci 203 3468-3475... 

Eritja R, Pons A, Escarceller M, et al. Synthesis of defined peptide-oligonucleotide hybrids containing a nuclear transport signal sequence. Tetrahedron 1991 47 4113-4120. 

Simmering, R., Kleessen, B., and Blaut, M., Quantification of the flavonoid-degrading bacterium Eubacterium ramulus in human fecal samples with a species-specific oligonucleotide hybridization probe, Appl. Environ. Microbiol, 65, 3705, 1999. 

P. Kumar, R.C. Willson, J.J. Valdes and J.P. Chambers, Monitoring of oligonucleotide hybridization using light-addressable potentiometric and evanescent wave fluorescence sensing, Mater. Sci. Eng. C, 1(3) (1994) 187-192. 

U. Maskos, E.M. Southern, Oligonucleotide Hybridizations on Glass Supports A Novel Linker for Oligonucleotide Synthesis and Hybridization Properties of Ohgonucleotides Synthesised In Situ , Nucl. Acids Res., 20(7), 1679-1684 (1992). 

An advantage of the ASO method is that it can be used to simultaneously test samples for several different mutations by the use of multiple probes bound to a solid matrix. In practice, the success of this method relies on precisely establishing conditions for optimal oligonucleotide hybridization in order to ensure specific probe hybridization, and so multiplex ASO assays can be difficult to develop. Molecular diagnostic kits for use in genetic disorders based on ASO methods are available (14). 

The choice of an oligonucleotide library came from the notion that oligonucleotide hybridization can be calculated accurately, even for large... 

With the features of field-enhanced, quadratic signal amplification and its self-referencing property [16-18], SPDS has shown improved sensitivity compared to the normal SPR in biosensing applications. Especially, it generates a significantly improved signal-to-noise ratio in the study of oligonucleotide hybridization, where a classical SPR sensor is usually poorly qualified. 

Selection of Oligonucleotide Hybridization Probes. The shortest possi-... 

The underlying premise of microarray analyses is oligonucleotide hybridizations that are used to gain genomic profiles of individual samples. These profiles can be... 

Table III shows that the luminescent lifetimes and the relative luminescent intensities for the covalently bound duplex and its noncovalent analogue are similar. As with [Ru(phen)2(dppz)]2+, a biexponential decay in emission is observed for the ruthenated oligonucleotide hybridized to its complement. A small shift in the wavelength of maximum emission is also observed compared to the noncovalent complex. This shift likely reflects the sensitivity in emission to the stacking of the oriented dppz ligand a dependence of the maximum emission wavelength on base...

Moore P, Clayton J (2003) To affinity and beyond. Nature 426 725-731 Budach W, Abel AP, Bruno AP et al (1999) Planar waveguides as high performance sensing platforms for fluorescence-based multiplexed oligonucleotide hybridization assays. Anal Chem 71 3347-3355... 

Du, X. Oligonucleotide hybridization on surfaces. Ph. D. Thesis, 1995, (Baylor College of Medicine). 

The specific way that SNPs can be identified varies between different brands. The most common ones are allele discrimination by hybridization in Affymetrix arrays (56) and allele-specific extension and ligation to a bar-code oligonucleotide hybridized to a universal array, as is done by the Illumina GoldenGate BeadArray Assay (57). 

Maskos U, Southern EM (1992) Oligonucleotide hybridizations on glass supports a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ. Nucleic Acids Res 20(7) 1679-1684... 

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