Species specificity oligonucleotides

Simmering, R., Kleessen, B., and Blaut, M., Quantification of the flavonoid-degrading bacterium Eubacterium ramulus in human fecal samples with a species-specific oligonucleotide hybridization probe, Appl. Environ. Microbiol, 65, 3705, 1999. 

Back, W., Bohak, L, Ehrmann, M., Ludwig, W., Schleifer, K. H. (1996). Revival of the species Lactobacillus lindneri and the design of a species specific oligonucleotide probe. Systematic and Applied Microbiology, 19, 322-325. 

As described by Williams et al. (1990), random amplified polymorphic DNA-PCR (RAPD-PCR) is a variant of PGR that utilizes oligonucleotide probes (9 to 12 base pairs or bp) to amplify several regions of the genome. The amplification products are then separated electrophoretically. Resolution depends upon the primer sequence and reaction conditions. RAPD-PCR can be made more specific by use of highly specific oligonucleotide probes. Holt and Cote (1998) applied this technique toward the identification of dextran-producing Oenococcus strains, and Esteve-Zarzoso et al. (1998) were able to identify Saccharomyces and Zygosaccharomyces species. Quesada and Cenis (1995) used the method to characterize wine yeasts. 

Kandimalla ER, Bhagat L, Zhu F-G et al (2003) A dinucleotide motif in oligonucleotides shows potent immunomodulatory activity and overrides species specific recognition observed with CpG motif. Proc Natl Acad Sci USA 100 14303-14308... 

Shikov, A. N. Temovoi, V. A. Sementsova, A. O. Agafonov, A. R Sergeev, A. N. Set of oligonucleotide primers and fluorescent-marked probes for species-specific instant identification of marburg virus by polymerase chain reaction. Russ. RU 2458143, 2012. 

RNA Fluorescent oligonucleotides 16S rRNA probes, specific for a species, genera or kingdom 118... 

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