Hybridization studies oligonucleotide

O. G. PNA-related oligonucleotide mimics and their evaluation for nucleic acid hybridization studies and analysis. Nucleosides, Nucleotides Nucleic Acids 2001 20 419-428. 

Using hybridization in situ with oligonucleotide probes, expression patterns of the five known kainate-type glutamate receptor subunit genes, KA1, KA2 and GluR5 to 7, have been discovered in brain tissue of adult and developing rat brain (Michaelis, 1998 Lerma et al., 2001). In situ hybridization studies also indicate that neurons showing the KA1 response are almost exclusively restricted to the CA3... 

The avidin-biotin procedure has been extensively used in hybridization studies. Since the concentration of all analysed DNAs used is identical, the total concentration of the analysed samples is high. The amplification of the base-mismatch recognition event is necessary to improve sensitivity. The use of oligonucleotide-functionahzed Uposomes or biotin-labelled liposomes as probes for the dendritic amphfication of DNA-sensing processes was characterized by Willner and co-workers [62] and showed better performance using QCM than impedance spectroscopy measurements. 

Baker et al. [95] were the first to report the formation of DNA-SWCNT adducts in solution for DNA hybridization. The DNA-SWCNT complexes were synthesized by reacting oxidized SWCNTs with thionyl chloride and ethylenediamine to form amine-terminated sites, shown in Fig. 14.16. The amines were further reacted with succinimidyl 4-(Af-maleimidomethyl)cyclohexane-l-carboxylate (SMCC) forming maleimide groups which reacted with thiol-modified DNA. To confirm covalent attachment and to test the accessibility of the DNA-modified SWNTs, hybridization studies were conducted using fluorescently labeled DNA oligonucleotide targets. Shortly thereafter, the use of carbodiimide-assisted coupling... 

HiUiard, L., Zhao, X., and Tan, W., Immobilization of oligonucleotides onto silica nanoparticles for DNA hybridization studies. Anal. Chim. Acta, 2002,470,51-56. 

Figure 11. Various sur ice architectures and experimental schemes for surface hybridization studies (a) a chromophore-Iabeled analyte hybridizes to an unlabeled sur ce-attached probe oligonucleotide strand (b) a chromophone-labeled, sur ce-ottached probe strand stretches upon hybridizing to an unlabeled target analyte strand, thus dacii the chtomophore fi er away (by Ad) from the quenching metal sur e (c) scheme firr a fluoiescence resonance energy tiansfer (FRET) experiment between a donor-dye labeled sur ce-attached probe oligonucleotide strand and an iuxteptor-dye labeled analyte target strand hybridized from solution.

Immobilization of oligonucleotides onto silica nanopartides for DNA hybridization studies. Analytica Chimica Acta, 470, 51-6. 

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