Surface-enhanced fluorescence , plasmonic

The Role Of Plasmonic Engineering In Potential Surface-Enhanced Fluorescence... 

The increased power of modern computers and the extension to semi-empirical methods for the molecular response [55] allows to routinely perform with this approach systematic studies of molecular plasmonics phenomena involving many different nanoparticle specimens, sizes and relative molecular positions/orientations [56-58], An example, for surface-enhanced fluorescence, is given in Fig. 5.10 see also Chapter 8. 

The unique photonic and condnctive properties of some nanoparticles can be employed to detect biological recognitions on a surface. With dimensions similar to those of biomolecnles, nanoparlicles are a natural choice for detecting biomolecules, which can be nsed in both electrochemically and optically based biosensors. Upon the assembly of nanoparticles on a solid surface, the biomolecules adsorbed on the surfaces of nanoparlicles can be detected by means of surface plasmon resonance (SPR), surface enhanced Raman spectroscopy (SERS), and surface-enhanced fluorescence spectroscopic techniques. Furthermore, the unique size-controlled optical properties of semiconductor nanoparticles imply that the organization of combinatorial hbraries of biomolecule-semiconductor nanoparticle hybrid systems or the assembly of these hybrids in array configurations may lead to the high-throughput parallel analysis of numerous analytes [53]. 

Liebermann T, Knoll W (2003) Parallel multispot detection of target hybridization to surface-bound probe oligonucleotides of different base mismatch by surface-plasmon field-enhanced fluorescence microscopy. Langmuir 9 1567-1572... 

Robelek R, Stefani FD, Knoll W (2006) Oligonucleotide hybridization monitored by surface plasmon enhanced fluorescence spectroscopy with bio-conjugated core/shell quantum dots. Influence of luminescence blinking. Phys Status Solidi A-Appl Mater Sci 203 3468-3475... 

Figure 5.44 (a) General molecular structure of the porphyrin disulfides, PDSn, described by Ishida and Majima [76]. (b) Changes in the surface plasmon enhanced fluorescence spectra for the exchange reaction of a decane thiol SAM with a 50 mmol dm-31,2-dichloroethane solution of PDS10 (Xem = 725 nm Xex = 425 nm). From A. Ishida and T. Majima, /. Chem. Soc., Chem. Commun., 1299-1300 (1999). Reproduced by permission of The Royal Society of Chemistry... 

Alternatively, various analytical methods based on SPR phenomenon have been developed, including surface plasmon field-enhanced Raman scattering (SERS) [7], surface plasmon field-enhanced fluorescence spectroscopy (SPFS) [8-11], surface enhanced second harmonic generation (SHG) [12], surface enhanced infrared absorption (SEIRA) [13], surface plasmon field-enhanced diffraction spectroscopy (SPDS) [14-18], Most of these methods take advantage of the greatly enhanced electromagnetic field of surface plasmon waves, in order to excite a chromophoric molecule, e.g., a Raman molecule or a fluorescent dye. Therefore, a better sensitivity is expected. 

Surface Plasmon Field-Enhanced Fluorescence Spectroscopy... 

Fig. 13. (A) Schematic representation of the interaction between neighboring residues of the His-tag (6 consecutive Histidine residues) and an NTA-complexed Ni2+ ion. (B) The performance of LHCII immobilization via chelating interaction. SPR kinetic curve of LHCII immobilization and regeneration cycles, monitored with an Nd YAG DPSS laser (X = 473 nm). (C) Surface plasmon field-enhanced fluorescence emission spectrum of surface attached LHCII compared with the fluorescence emission from free LHCII in solution, excited by an Nd YAG DPSS laser (X — 473 nm).

Chu L-Q, Forch R, Knoll W (2007) Surface-plasmon-enhanced fluorescence spectroscopy for DNA detection using fluorescently labeled PNA as DNA Indicator . Angew Chem Int Ed 46 4944-4947... 

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