Oligonucleotide hybridization colorimetric detection

Chapters 17-22 describe the hybridization of the nonradioactive probes to the DNA and RNA immobilized on blots, together with the detection systems necessary to reveal where the probe has hybridized. Chapters 17-19 deal with digoxigenin probes, with Chapters 17 and 19 describing chemiluminescent detection on DNA and RNA blots respectively, and Chapter 18 describing a colorimetric detection system. Chapter 20 deals with enhanced chemiluminescent detection of enzymically labeled probes, whereas Chapters 21 and 22 describe enhanced chemiluminescent detection of large (Chapter 21) and small (oligonucleotide. Chapter 22) probes labeled with fluorescein. 

The dependence of the plasmon band on the dielectric constant of the surrounding medium in metal nanocrystals had been used to detect binding events taking place at the ligand shell. Thus, Au nanocrystals could colorimetrically determine the successful hybridization of oligonucleotide strands bound to its surface [83, 86]. It has been proposed that colorimetric sensing of heavy metal ions could be obtained by the use of carboxylic acid terminated bifunctional... 

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