Oligonucleotide complexes

Zelphati O, et al. Effect of serum components on the physico-chemical properties of cationic lipid/oligonucleotide complexes and on their interactions with cells. Biochim Biophys Acta 1998 1390 119. 

De Pauw, E. Determination of binding constants of oligonucleotide complexes with minor groove binders by electrospray ionization mass spectrometry. Comparison with fluorescence titration data. Adv. Mass Spectrom. 2001, 15, 795-796. 

Bugaut, A. Toulme, J.-J. Rayner, B. Use of dynamic combinatorial chemistry for the identification of covalently appended residues that stabilize oligonucleotide complexes. Angew. Chem. Int. Ed. 2004,43, 3144-3147. 

Nerdal, W., Andersen, 0.M., and Sletten, E., NMR studies of a plant flavonoid — DNA oligonucleotide complex, Acta Chem. Scand, 47, 658, 1993. 

Similar to pDNA, the pharmacokinetics of oligonucleotides can be controlled by using delivery systems. Mahato et al. (1997) studied the pharmacokinetic characteristics of antisense oligonucleotides complexed with Gal-PLL or mannosylated PLL (Man-PLL), whose mannose residues can be recognized by mannose... 

Mahato, R.I., Takemura, S., Akamatsu, K., Nishikawa, M., Takakura, Y. and Hashida, M. (1997) Physicochemical and disposition characteristics of antisense oligonucleotides complexed with glycosylated poly(L-lysine). Biochem. Pharmacol., 53, 887-895. 

An ESI mass spectrum of a mixture of 1 1 d(pT)13 d(pA)14 with the gene V protein (Fig. 13a) shows the existence of a double-stranded dimer and of a complex between the protein and a single d(pT)13 oligonucleotide. Complexes of the protein with the dsDNA, or with d(pA)14 do not appear in the spectrum. This clearly points to the preference for binding ssDNA over dsDNA and poly(dT) over polyfdA). When the mixture of d(pT)13 d(pT)15 ... 

Information on molecular recognition is also found in the crystal structures of proteins or oligonucleotides complexed with small molecules. 

Oligonucleotide complex stability has also been enhanced with cationic deoxynucleic and ribonucleic guanidine backbones (DNG and RNG) (Figure 127), which are electrostatically attracted to the anionic phosphodiester backbone of natural chains within a complex (substituted guanidinium linkages have also been investigated715). Through both solution-716-718 and solid-phase synthesis,719 722 pentameric DNG T... 

Figure 14 Electrospray mass spectra at a fixed concentration of oligonucleotide and four concentrations of distamycin. The spectra show the uncom-plexed oligonucleotide at both the -7 and -8 charge states and the appearance of the distamycin oligonucleotide complex at higher concentrations of the ligand (distamycin = DstA).

More recently, the same group attempted to deliver an antisense oligonucleotide complexed with folate liposomes in a series of in vitro and in vivo experiments [69]. Although oligonucleotide-loaded folate liposomes were effectively associated with KB cells... 

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