Polypeptide-oligonucleotide complexe

Biosynthetic polypeptide libraries are the translation of a genetic, oUgonucleotide-based diversity information into a biologically relevant pool of p)otential binders, catalysts, or receptors. The previous section illustrated how the availability of complex DNA sequences and of the appropriate techniques for their synthesis and manipulation allows the biological production of diverse polypeptide libraries. However, the intrinsic properties of nucleotide sequences have themselves stimulated researchers into budding a variety of oligonucleotide (ON) libraries per se for a variety of applications. 

The object of this chapter is to provide some background for the appreciation of the pharmaceutics of proteins, peptides DNA, oligonucleotides and monoclonal antibodies as therapeutic entities. From an understanding of the nature of amino acids and their physical properties comes an appreciation of the physical nature and properties of peptides, polypeptides and proteins, defined below. The solution properties of proteins in simple and complex media should be understood, together with the factors affecting the stability of proteins in solution. Problems in the formulation of proteins to be overcome will then become clearer. 

---