Olfactory receptor neurons turnover

Ronnet G. V., Cho H., Hester L. D., Wood S. F. and Snyder S. H. (1993) Odorants differentially enhance phosphoinosite turnover and adenylyl cyclase in olfactory receptor neuronal cultures. J. Neurosci. 13, 1751-1758. 

Ingi, T., Chiang, G., Ronnett, G.V. (1996). The regulation of heme turnover and carbon monoxide biosynthesis in cultured primary rat olfactory receptor neurons. J. Neurosci. 16 5621-8. 

Sandeman RE, Sandeman DC (1996) Pre-and postembryonic development, growth and turnover of olfactory receptor neurons in crayfish antennules. J Exp Biol 199 2409-2418... 

Very recent efforts to further evaluate the functional implications of phosphoinositol signaling in vertebrate chemosensory transduction have led to the discovery that not only the phosphoinositol breakdown pathway catalyzed by phospholipase C, but also membrane phosphoinositols themselves involving phosphoinositide-3-kinase may play a functional role in the transduction process. It was found that 3-phosphoinositides signaling in concert with the canonical phosphoinositide turnover pathway modulate the cyclic nucleotide signaling cascade downstream of the receptor. The data suggest that 3-phosphoinositide, the primary product of PI3K activity, attenuates the cyclic nucleotide-dependent excitation of olfactory neurons (Spehr et al., 2002). 

Since one of the major functions of IP3 is to release Ca from intracellular stores, the issue of whether chemoattractant binding to its receptor increased intracellular calcium was addressed (Cinelli et al., 2002). A calcium sensitive dye. Calcium green 1, was injected into the accessory olfactory bulb of snakes. The animals survived 4-6 days to permit retrograde transport of the dye to the cell bodies of receptor cells in VN sensory epithelium. Thus, in VN sensory epithelial slices ( 240 pm thick) only mature VN neurons were stained with the calcium-sensitive dye and this population gave rise to the Ca increases in response to ESS, bradykinin (a stimulator of phosphoinositide turnover) and forskolin (an adenylate cyclase activator). 

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