Nucleotides oligonucleotide syntheses

Nucleophiles. See Donor synthons Nucleotides oligonucleotide syntheses, 215-224, 341-343... 

Nucleotides and nucleic acids are critical tools in the areas of gene expression, therapeutics, and diagnostics. However, there are certain challenges associated with their large-scale purification and subsequent characterization. While solid-state oligonucleotide synthesis is relatively simple and can be totally automated, intra- and intermolecular associations may occur involving shorter sequences that may hybridize with the desired full length... 

Saladino R, Crestini C, Costanzo G, DiMauro E (2005) On the Prebiotic Synthesis of Nucle-obases, Nucleotides, Oligonucleotides, Pre-RNA and Pre-DNA Molecules. 259 29-68 Santos I, Paulo A, Correia JDG (2005) Rhenium and Technetium Complexes Anchored by Phosphines and Scorpionates for Radiopharmaceutical Applications. 252 45-84 Santos M, see Szathmdry E (2005) 259 167-211... 

It is still unclear how much general validity attaches to the metaphosphate mechanism as formulated for the present variant for the oligonucleotide synthesis. Depending upon the reaction conditions, preparative method, activating reagent, and length of the nucleotide block, other mechanisms may dominate. 

A supported sulfide source has been demonstrated by Zhang et al.16 to synthesize oligonucleotide phosphorothioates (entry 12). Aminodithiazole-thione attached to a methacrylate-ethyleneglycol copolymer is used to efficiently convert a nucleotide phosphite to a phosphorothioate. The product nucleotide possesses protecting groups suitable for solid-phase oligonucleotide synthesis and thus is a valuable building block for nucleic acid therapeutics. 

On the Prebiotic Synthesis of Nudeobases, Nucleotides, Oligonucleotides, Pre-RNA and Pre-DNA Molecules... 

Keywords Origin of life Prebiotic synthesis Nucleic bases Nucleotides Oligonucleotides... 

Saladino R, Crestini C, Costanzo G, DiMauro E. On the prebiotic synthesis of nucleo-bases, nucleotides, oligonucleotides, pre-RNA and pre-DNA molecules. Top Curr Chem 2005 259 29-68. 

Donga RA, Hassler M, Chan T-K, Damha MJ. Oligonucleotide synthesis using ionic liquids as soluble supports. Nucleosides Nucleotides Nucleic Acids 2007 26 1287-1293. 

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