Solid-phase synthesis oligonucleotides

To enable a covalent labeling of target molecules, the dyes are functionalized with reactive groups. These are either phosphoramidites for the labeling of synthetic oligonucleotides (solid-phase synthesis), or uridine-5 -triphosphates for enzymatic coupling in PCR (random primed labeling... 

Hurley, D. J., Tor, Y. (1998). Metal-containing oligonucleotides Solid-phase synthesis and luminescence properties, J. Am. Chem. Soc., 120 2194. 

Taylor JS, Brockie IR, O Day CL (1987) A building block for the sequence-specific introduction of ds-syn thymine dimers into oligonucleotides, solid-phase synthesis of TpT[c,s]pTpT. J Am Chem Soc 109 6735... 

One widely used method involving protected compounds is solid-phase synthesis (polymer-supponed reagents). This method has the advantage of requiring only a simple workup by filtration such as in automated syntheses, especially of polypeptides, oligonucleotides, and oligosaccharides. 

Synthetic oligonucleotides are very important tools in the study and manipulation of DNA, including such techniques as site-directed mutagenesis and DNA amplification by the polymerase chain reaction. The techniques for chemical synthesis of oligonucleotides are highly developed. Very efficient automated methodologies based on solid phase synthesis are used extensively in fields that depend on the availability of defined DNA sequences.52... 

Over the past decade the techniques of combinatorial synthesis have received much attention. Solid phase synthesis of polypeptides and oligonucleotides are especially adaptable to combinatorial synthesis, but the method is not limited to these fields. The goal of combinatorial synthesis is to prepare a large number of related... 

With the development of appropriate cartridges and proper chemistry that would allow the synthesis of the molecule on the disk and than the use of the same disk directly for affinity chromatography by placing it in a chromatographic cartridge, it is reasonable to expect that SMC will become a very efficient tool in the solid phase synthesis of peptides, oligonucleotides, and similar molecules. 

The ability to synthesize chemically short sequences of single-stranded DNA (oligonucleotides) is an essential part of many aspects of genetic engineering. The method most frequently employed is that of solid-phase synthesis, where the basic philosophy is the same as that in solid-phase peptide synthesis (see Section 13.6.3). In other words, the growing nucleic acid is attached to a suitable solid support, protected nucleotides are supplied in the appropriate sequence, and each addition is followed by repeated coupling and deprotection cycles. 

Scheme 13.55. Automated Solid-Phase Synthesis at Oligonucleotides ...

Likewise, in the preparation of many ion-exchange resins, suitable functional groups are introduced by secondary reactions of macromolecular substances (that are generally crosslinked see Sect. 5.2). In this context the utilization of crosslinked polystyrene resins or poly(acrylamide) gel in the solid-phase synthesis of polypeptides (Merrifield technique) or even oligonucleotides should be mentioned. After complete preparation of the desired products they are cleaved from the crosslinked substrate and can be isolated. 

Hruby, V. J. Al-Obeidi, F. Sanderson, D. G. Smith, D. D., In Innovations and Perspectives in Solid Phase Synthesis Peptides, Polypeptides and Oligonucleotides, Epton, R., Ed. ... 

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