Nucleotide sequence of gene

Sato, S-I, N Ouchiyama, T Kunura, H Nojiri, H Yamane, T Omori (1997a) Cloning of gees involved in carbazole degradation of Pseudomonas sp. strain CAIO nucleotide sequences of genes and characterization of meta cleavage enzymes and hydrolase. J Bacteriol 179 4841-4849. 

Generic Recombination, made possible by the discovery of the recombinant DNA procedure in the 1970s, makes it possible to develop extensive and detailed maps of the nucleotide sequences of gene molecules—to the point where, in 1990. plans were outlined for mapping the complete human genome, a program that wax well underway as of 1994. 

Yamawaki-Rataoka, Y., Nakai, S., Miyata, T., Honjo, T. (1982). Nucleotide sequences of gene segments encoding membrane domains of immunoglobulin y chains. Proc. Natl. Acad. Sci. USA 79, 2623-2627. 

A series of incisive studies in the late 1950s and early 1960s revealed that the amino acid sequences of proteins are determined by the nucleotide sequences of genes. The sequence of nucleotides in DNA specifies a complementary sequence of nucleotides in RNA, which in turn specifies the amino acid sequence of a protein. In particular, each of the 20 amino acids of the repertoire is encoded by one or more specific sequences of three nucleotides (Section 5,5). 

Brisson-Noel, A., and Courvalin, P. (1986). Nucleotide sequence of gene linA encoding resistance to lincosamide in Streptococcus haemolyticus. Gene 43, 247-253. 

Rince A, Dufour A, Le Pogam S, Thuault D, Bourgeois CM, Le Pennec JP (1994) Cloning, expression and nucleotide sequence of genes involved in production of lactococcin DR, a bacteriocin from lactococcus lactis subsp. lactis. Appl Environ Microbiol 60 1652-1657... 

Yuuki T, Nomura T, Tezuka H, Tsuboi A, Tsukagoshi H, Yamagata N, Udaka S (1985) Complete nucleotide sequence of gene coding for heat-and pH-stable alpha-amylase of Bacillus licheniformis comparison of the amino acid sequence of 3 bacterial liquefying alpha-amylases deduced from the DNA. JBiochem 98 1147-1156... 

The amount of sample required is quite small as little as 10 mole is typical So many peptides and proteins have been sequenced now that it is impossible to give an accurate count What was Nobel Prize winning work m 1958 is routine today Nor has the story ended Sequencing of nucleic acids has advanced so dramatically that it is possible to clone the gene that codes for a particular protein sequence its DNA and deduce the structure of the protein from the nucleotide sequence of the DNA We 11 have more to say about DNA sequencing m the next chapter... 

After a desired clone is obtained and mapped with restriction enzymes, further analysis usually depends on the deterrnination of its nucleotide sequence. The nucleotide sequence of a new gene often provides clues to its function and the stmcture of the gene product. Additionally, the DNA sequence of a gene provides a guidepost for further manipulation of the sequence, for example, lea ding to the production of a recombinant protein in bacteria. 

Streptokinase has a molecular weight of about 47,000 with a single chain of 415 amino acids there are no intramolecular disulfide bonds (64). The complete nucleotide sequence of the gene encoding the RNA for this protein has been reported (65,66). 

All protein molecules are polymers built up from 20 different amino acids linked end-to-end by peptide bonds. The function of every protein molecule depends on its three-dimensional structure, which in turn is determined by its amino acid sequence, which in turn is determined by the nucleotide sequence of the structural gene. 

Cohn, D. H., et al. (1985). Nucleotide sequence of the luxA gene of Vibrio harveyi and the complete amino acid sequence of the a subunit of bacterial luciferase. J. Biol. Chem. 260 6139-6146. 

Foran, D. R., and Brown, W. M. (1988). Nucleotide sequence of the LuxA and LuxB genes of the bioluminescent marine bacterium Vibrio fischeri. Nucleic Acids Res. 16 111. 

Johnston, T. C., et al. (1990). The nucleotide sequence of the luxA and luxB genes of Xenorhabdus luminescence HM and a comparison of the amino acid sequences of luciferases from four species of bioluminescent bacteria. Biochem. Biophys. Res. Commun. 170 407- 115. 

Diaz-De-Leon F. Klotz K.L. Lagrimini L.M (1993) Nucleotide sequence of the tobacco Nicotiana tabacum) anionic peroxidase gene / / Plant Physiol. V. 101. P. 1117-1118. 

The genetic information within the nucleotide sequence of DNA is transcribed in the nucleus into the specific nucleotide sequence of an RNA molecule. The sequence of nucleotides in the RNA transcript is complementary to the nucleotide sequence of the template strand of its gene in accordance with the base-pairing rules. Several different classes of RNA combine to direct the synthesis of proteins. 

If the nucleotide sequence of the gene containing the mutation is transcribed into an RNA molecule, then the RNA molecule will possess a complementary base change at this corresponding locus. 

Yanisch-Perron, C., Vieira, J. and Messing, J. 1985. Improved M13 phage cloning vectors and host strains nucleotide sequences of the M13mpl8 and pUCl9 vectors. Gene 33 103-1 19. 

Molecular cloning and nucleotide. sequencing of amino acid permease genes... 

D-Aminoacid oxidase has been isolated from a nnmber of yeasts, and the nucleotide sequence of the enzyme from Rhodotorula gracilis ATCC 26217 has been established (Alonso et al. 1998). The gene could be overexpressed in Escherichia coli, and levels of the enzyme were greater under conditions of low aeration the enzyme isolated from the recombinant organisms was apparently the apoenzyme since maximum activity required the presence of FAD. 

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