LuxA gene

Cohn, D. H., et al. (1985). Nucleotide sequence of the luxA gene of Vibrio harveyi and the complete amino acid sequence of the a subunit of bacterial luciferase. J. Biol. Chem. 260 6139-6146. 

The primer luxAF03 locates 261 bp downstream from the 3 end of luxAF05. Sequencing and RFLP analysis of the luxA genes... 

Table 1. Summary of the RFLP analysis of the luxA genes in the symbiotic P. leiognathi isolates from L. rivulatus collected in 2002.

Baldwin, T. O., et al. (1987). Applications of the cloned bacterial luciferase genes luxA and luxB to the study of transcriptional promoters and terminators. In Schoelmerich, J. (ed.), Biolumin. Chemilumin., Proc. Int. Biolumin. Chemilumin. Symp., 4th, 1986, pp. 373-376. Wiley, Chichester, UK. 

Foran, D. R., and Brown, W. M. (1988). Nucleotide sequence of the LuxA and LuxB genes of the bioluminescent marine bacterium Vibrio fischeri. Nucleic Acids Res. 16 111. 

Johnston, T. C., et al. (1990). The nucleotide sequence of the luxA and luxB genes of Xenorhabdus luminescence HM and a comparison of the amino acid sequences of luciferases from four species of bioluminescent bacteria. Biochem. Biophys. Res. Commun. 170 407- 115. 

Gupta RK, Patterson SS, Ripp S, Simpson ML, Sayler GS. Expression of the Photo-rhabdus luminescens lux genes luxA, B, C, D, and E) in Saccharomyces cerevisiae. FEMS Yeast Res 2003 4 305-313. 

Two types of ludferase genes, cloned from bacteria and firefly, are used as sensitive reporter systems in a wide variety of cells such as barterial, yeast, insect, animal, and plant cells. Bacterial luciferases are flavoenzymes composed of two subunits each encoded by the luxA and luxB genes, while the firefly ludferase is a single polypeptide spedfied by the luc gene. The two types of luciferase catalyze... 

The luxA and luxB genes of V. harveyi are contained within a 4.0-kbp HmdIII genomic fragment and are immediately adjacent to each other (44). 

The luxB gene comprises 324 codons and, like the luxA, terminates with UAA. The stop codon is followed by a 27-bp spacer before the start of luxE, A 20-bp region of a potential stem-loop structure is located 3 to the luxB ORF and that includes the 5 -proximal 9 bases of luxE (32). 

The intensity of light emission in most luminescent marine bacteria and in other bacteria harboring the cloned luxA and luxB genes depends on a number of parameters, including the cAMP system and, in particular, the density of the cell suspension (43). In fact, it is not the cell density per se that directly influences... 

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